BioXCell热销产品–RecombiMAb anti-mouse CSF1R (CD115)
BioXCell热销产品–RecombiMAb anti-mouse CSF1R (CD115)
产品描述：
AFS98-CP131单克隆抗体是原始AFS98单克隆抗体的重组嵌合型抗体。可变结构域序列与原始AFS98单克隆抗体相同，但是恒定区序列已经从大鼠IgG2a变到小鼠IgG2a。AFS98-CP131单克隆抗体像原始大鼠IgG2a抗体一样，不包含Fc突变。
AFS98-CP131单克隆抗体能与小鼠集落刺激因子1受体(CSF1R)，也称为巨噬细胞集落刺激因子受体(M-CSFR)，即CD115反应。CSF1R是一种单程I型膜蛋白，是血小板衍生生长因子受体家族的成员。在小鼠中，CSF1R由单核细胞/巨噬细胞、腹膜渗出物细胞、浆细胞样和常规树突细胞以及破骨细胞表达。CSF1R是CSF1的受体，CSF1通过CSF1R调节单核细胞谱系中细胞的增殖和分化。据报道，AFS98单克隆抗体可在体内deplete巨噬细胞并阻断CSFR1。
产品详情：
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产品名称
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RecombiMAb anti-mouse CSF1R (CD115)
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产品货号
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CP131
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产品规格
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1/5/25/50/100mg
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反应种属
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Mouse
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克隆号
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AFS98-CP131
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同种型
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Mouse IgG2a(switched from rat IgG2a)
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免疫原
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Not available or unknown
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实验应用
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in vivo macrophage depletion*
in vitro CSF1R neutralization*
in vivo monocyte depletion*
Flow cytometry*
Western blot*
*Reported for the original rat IgG2a AFS98 antibody
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产品形式
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PBS, pH 7.0,Contains no stabilizers or preservatives
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纯度
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>95%, Determined by SDS-PAGE
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聚合
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<5%, Determined by SEC
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无菌处理
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0.2 µm filtration
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纯化方式
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Protein A
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分子量
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150 kDa
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小鼠病原检测
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Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
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保存条件
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抗体原液保存在4°C，不能冷冻保存。
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推荐同型对照
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InVivoPlus mouse IgG2a isotype control, unknown specificity（货号BP0085）
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推荐抗体稀释液
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InVivoPure pH 7.0 Dilution Buffer（货号IP0070）
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该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：
| 应用 |
文章
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体内巨噬细胞耗竭
(in vivo macrophage
depletion)
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1. Bauche, D., et al. (2018). ‘LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing
CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis’
Immunity 49(2): 342-352 e345.
2. Gordon, S. R., et al. (2017). ‘PD-1 expression by tumour-associated macrophages inhibits
phagocytosis and tumour immunity’ Nature 545(7655): 495-499.
3. Moynihan, K. D., et al. (2016). ‘Eradication of large established tumors in mice by combination
immunotherapy that engages innate and adaptive immune responses’ Nat Med. doi : 10.1038/nm.4200.
4. Naik, S., et al. (2015). ‘Commensal-dendritic-cell interaction specifies a unique protective
skin immune signature’ Nature 520(7545): 104-108.
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体外CSF-R1中和
(in vitro CSF-R1
neutralization)
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1.Sheng, K. C., et al. (2014). ‘IL-3 and CSF-1 interact to promote generation of
CD11c+IL-10-producing macrophages’ PLoS One 9(4): e95208.
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流式细胞术
(Flow Cytometry)
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1. Li, W., et al. (2012). ‘Intravital 2-photon imaging of leukocyte trafficking in beating
heart’ J Clin Invest 122(7): 2499-2508.
2. Tagliani, E., et al. (2011). ‘Coordinate regulation of tissue macrophage and dendritic
cell population dynamics by CSF-1′ J Exp Med 208(9): 1901-1916.
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更多产品详情请咨询 BioXCell 中国代理——上海金畔生物
