Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Small intestine (Mouse) Lysate at 30 ug
Primary: Anti-ZNF300(JP120084a) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution;
Predicted band size : 66kD
Observed band size : 70,66kD

ZNF259 Antibody (C-term) (Cat. #JP102876) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ZNF259 antibody detected the ZNF259 protein (arrow).

ZNF259 Antibody (C-term) (Cat. #JP102876) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the ZNF259 antibody detected the ZNF259 protein (arrow).

Anti-ZNF238 Antibody (C-Term) at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ZNF225 expression in rat kidney (A) whole cell lysates.

Immunohistochemical analysis of ZNF225 staining in human thymus gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ZNF217 Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ZNF217 Antibody (N-term) (Cat. #JP102692) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ZNF217 antibody detected the ZNF217 protein (arrow).

Western blot analysis of lysate from SH-SY5Y cell line, using ZNF219 Antibody (N-term)(Cat. #JP104077). JP104077 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of lysate from human heart tissue, using ZNF219 Antibody (N-term) (Cat. #JP104077). JP104077 was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of anti-ZNF219 Antibody (N-term) (Cat.#JP104077) in 293 cell line lysates (35ug/lane).ZNF219(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with ZNF219 antibody (N-term) (Cat.#JP104077), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ZNF227 Antibody (N-term ) (Cat. #JP101854) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ZNF227 antibody detected the ZNF227 protein (arrow).

ZNF227 Antibody (N-term) (Cat. #JP101854)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZNF227 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.