E169 MES FREE ACID MONOHYDRATE Grade:ULTRA PURE GRADE CAS Number: 145224-94-8Synonym(s): 2-(N-morpholino)ethanesulfonic acid Molecular Weight: 213.2Molecular Formula: C6H13NO4S.H2O Storage Condition: ROOM TEMPERATURE CodeSizePriceQuantity E169-100G E169-250G E169-500G Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes or biological membranes (penetration, solubilization, adsorption on surface, etc.), have known complex-forming tendency with metal ions, be non-toxic and have a very low U.V. absorption at wavelength >260 nm.
Guide-it SNP Screening Kit可以检测CRISPR/Cas9系统编辑后的细胞群中的单核苷酸替换,在96孔板中快速识别出基因编辑后的细胞克隆。整个操作流程简单快速,首先通过PCR扩增基因组靶标位点,之后使用识别特定结构的核酸内切酶进行酶切反应,然后读取荧光值就可以完成单核苷酸替换检测。无论需要检测的核苷酸替换类型、细胞克隆的接合性或者靶标位点的基因序列如何,都可以使用这个方法进行检测。了解此试剂盒的原理以及更多产品特点,您可以登录FAQs页面观看视频录像。
基因编辑技术其中一个强大的应用,就是在特定的基因组位点导入核苷酸替换,去模拟与人类疾病相关的单核苷酸多态性(SNPs)或者插入终止密码子以实现准确的基因敲除。然而,要从数以百计的克隆中筛选出一个对单核苷酸进行了编辑的细胞克隆,这对我们来说仍然是一个挑战,尤其是在没有相应的表型的情况下。而Guide-it SNP Screening Kit可以充分满足这个需求,让您在数百个细胞克隆中轻松筛选出单核苷酸编辑。
图2 比较纯合子、杂合子和野生型细胞样品的SNP筛选结果。分别以纯合子、杂合子和野生型细胞为样品,使用Guide-it SNP Screening Kit检测所标明的核苷酸替换。在每一组检测结果中,在纯合子和杂合子样品(分别为蓝色和紫色)中所检测到荧光信号值相差不大。
图3使用Guide-it SNP Screening Kit检测基因分型。本实验例中的SNPs样品均来自于Coriell研究所,这些样品在两个基因组位点(NCP1或者CFT基因)中的其中一个位点上携带了SNPs。使用Guide-it SNP Screening Kit(柱形图)和Sanger测序进行分析。Panel A. NCP1基因型分析。使用分别检测A或者G的两个flap-probe oligos独立进行实验,分析确定哪些样品是A>G替换的纯合子,哪些是杂合子。Panel B. CFTR基因型分析。分析确定哪些样品是没有发生T>A替换的野生型,哪些是T>A替换的纯合子。所有检测结果都通过Sanger测序进行了确认(结果显示在柱形图下面每个样品的下方)。
图4 利用Guide-it SNP Screening Kit在多个人类基因组位点检测核苷酸替换。以野生型或者纯合子来源的基因组DNA(从Coriell研究所获取)为样品,使用Guide-it SNP Screening Kit分析所标明的碱基替换。结果表明所有的碱基替换均可以被成功检测到,与野生型(橙色)和阴性对照(灰色)样品所获得的荧光信号相比,发生了所标明的核苷酸替换的纯合子(蓝色)样品产生了强荧光信号就可以说明这一点。
This kit is intended for use in quantifying very low concentrations of human immunoglobulin A. The antibodies used in this kit are very specific for hIgA, allowing for accurate quantitation even in the presence of other human immunoglobulins. The limit of detection is less than 150pg/mL.
PNPP Liquid Substrate F008 For use with Alk Phos in ELISA, 100mL
PNPP Liquid Substrate F10008 For use with Alk Phos in ELISA, 1L
TMB Liquid Substrate F027 For use with HRP in ELISA, 100mL
TMB Liquid Substrate F100027 For use with HRP in ELISA, 1L
TMB Liquid Substrate F129 For use with HRP in Western Blot, 100mL
TMB Liquid Substrate F100029 For use with HRP in Western Blot, 1L
BCIP/NBT Liquid Substrate F064 For use with Alk Phos in WB, 1 reagent, 100mL
BCIP/NBT Liquid Substrate F00064 For use with Alk Phos in WB, 1 reagent, 1L
Sample Dilution Buffers
Sample Dilution Buffer, 1L, 100mL, 500mL I028 I028-100 I028-500 For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits
A549 Sample Diluent, 100mL , 500mL F233A-100 F233A-500 For use with A549 HCP ELISA kit
BSA Sample Dilution Buffer, 1L , 100mL , 500mL F031A / F031A-100 / F031A-500 For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits
Goat IgG Dilution Buffer, 1L , 100mL , 500mL F213A /F213A-100 / F213A-500 For use with Goat IgG ELISA kit
Goat Milk Diluent Buffer, 1L , 100mL r, 500mL F243A /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit
NS/0 Sample Dilution Buffer, 1L ,100mL ,500mL F223A / F223A-100 /F223A-500 For use with NS/0 HCP ELISA kit
SP2/0 Sample Dilution Buffer, 1L , 100mL , 500mL F183A / F183A-100 / F183A-500 For use with SP2/0 HCP ELISA kit
Immunogenicity Products
Passive Coated Immunogenicity Kit IM10 Kit for immunogenicity testing
Biotinylated Antigen Coated Immunogenicity Kit IM20 Kit for immunogenicity testing
Biotinylation Kit IM30
Human Immunoglobulin Screening Kit IM40 Kit for immunogenicity testing
Human IgG Subclass Kit IM50 Kit for immunogenicity testing
Substrate Kit IM109 TMB & Stop Solution for ELISA, 50mL
Coatingate Buffer, 1L IM101 For immunogenicity testing
Conjugate Diluent, 1L IM103 For immunogenicity testing
Conjugate Diluent, 500mL IM103-500 For immunogenicity testing
Sample Diluent, 500mL IM104 For immunogenicity testing
FG Blocker, 1L IM107 For immunogenicity testing
BSA Antigen, 1mg/mL IM112 For immunogenicity testing
Bovine IgG Antigen, 1mg/mL IM113 For immunogenicity testing
Bovine Transferrin Antigen, 1mg/mL IM114 For immunogenicity testing
CHO HCP Antigen, 1mg/mL IM115 For immunogenicity testing
E. coli HCP Antigen, 1mg/mL IM116 For immunogenicity testing
Goat IgG Antigen, 1mg/mL IM117 For immunogenicity testing
HSA Antigen, 1mg/mL IM118 For immunogenicity testing
Human IgD Antigen, 1mg/mL IM141 For immunogenicity testing
Human Transferrin Antigen, 1mg/mL IM119 For immunogenicity testing
Insulin Antigen, 1mg/mL IM121 For immunogenicity testing
Mouse IgG Antigen, 1mg/mL IM122 For immunogenicity testing
NS/0 HCP Antigen, 1mg/mL IM123 For immunogenicity testing
SP2/0 HCP Antigen, 1mg/mL IM125 For immunogenicity testing
Gyros Kits-discontinued
CHO 3G HCP G550 For use on the Gyros xP workstation, 560 tests
CHO 3G HCP G550-1 For use on the Gyros xP workstation, 112 tests
E.coli HCP G410 For use on the Gyros xP workstation, 560 tests
E.coli HCP G410-1 For use on the Gyros xP workstation, 112 tests
Protein A G400 For use on the Gyros xP workstation, 560 tests
Protein A G400-1 For use on the Gyros xP workstation, 112 tests
DNA Kits
DNA Extracton in Wells D100W 96 tests in a deep well microtiter plate
DNA Extraction in Tubes D100T 50 tests in microfuge tubes
Residual CHO Host Cell kit in Wells D550W 96 tests in a deep well microtiter plate
Residual CHO Host Cell kit in Tubes D550T 50 tests in microfuge tubes
CHO Amplification kit in Wells D555W 96 tests in a deep well microtiter plate
CHO Amplification kit in Tubes D555T 50 tests in microfuge tubes
Residual Ecoli Host Cell kit in Wells D410W 96 tests in a deep well microtiter plate
Residual Ecoli Host Cell kit in Tubes D410T 50 tests in microfuge tubes
E.coli Amplification kit in Wells D415W 96 tests in a deep well microtiter plate
E.coli Amplification kit in Tubes D415T 50 tests in microfuge tubes
Residual NS/0 Host Cell kit in Wells D220W 96 tests in a deep well microtiter plate
Residual NS/0 Host Cell kit in Tubes D220T 50 tests in microfuge tubes
Miscellaneous Reagents & Components
Deep Well Extraction Plate w/Sealing Mat D102 5 plates