日度归档：2024年4月7日

小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y50035 Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit 1 Kit ￥23,991 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
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来源于人类iPS细胞的小肠上皮细胞
(Intestinal Epithelial Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis Intestinal Epithelial Cells (from ChiPSC18) 是来源于人iPS细胞的小肠上皮细胞，具备肠上皮样特征，包括形成紧密连接、表达绒毛蛋白villin和肠转录因子CDX2。与人结肠癌Caco-2细胞系相比，该细胞具有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达，为药物代谢吸收、药物相互作用评估提供更好的预测结果。
Cellartis Intestinal Epithelial Cells (from ChiPSC18) Kit 包括1管冻存的细胞Cellartis Intestinal Epithelial Cells (from ChiPSC18)和1个试剂盒Cellartis IEC Maturation Kit。解冻的Cellartis Intestinal Epithelial Cells (from ChiPSC18)使用Cellartis IEC Maturation Kit预先培养5天后，可用于各种检测。
 
■ 产品特点
· 解冻5天内细胞形成紧密连接，解冻5-8天后可用于检测
· 细胞高表达肠上皮标志物 (villin、CDX2等)
· 与Caco-2细胞相比，拥有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达
· 细胞形成一个功能性的、可渗透的屏障，可用于预测药物在体内的渗透性和吸收
 
■ 产品应用
· 药物在小肠的吸收和代谢评价
· 药物研发中的ADME分析
· 药物渗透性检测
 
■ 产品组成
· 1 vial of～4.8 x 106 cells
· 1 Cellartis IEC Maturation Kit（IEC Maturation Basal Medium；IEC Supplement A；IEC Supplement B；IEC Supplement C；IEC Supplement D）
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
 
实验例
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图1. 紧密连接蛋白ZO-1的免疫荧光染色结果显示，解冻后第5天，hiPSC-derived intestinal epithelial cells (IEC)细胞间形成紧密连接（如闭锁小带zonula occludens）。
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图2. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达肠上皮标志物villin和CDX2。
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图3. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达药物代谢酶CYP3A4和药物转运蛋白PEPT1。
 
 
■ 产品详情请点击：小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
 
 

页面更新：2024-02-29 10:53:34

胰腺β细胞Human Stem Cell Derived Beta Cells酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

胰腺β细胞Human Stem Cell Derived Beta Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y10100 Cellartis® hiPS Beta Cells (from ChiPSC12) Kit 1 Kit 询价 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells
Cellartis Y10106 Cellartis® hiPS Beta Cells (from ChiPSC22) Kit 1 Kit ￥25,928 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells
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来源于人类iPS细胞的胰腺β细胞
(Pancreatic Beta Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis® hiPS Beta Cells Kit是由hiPS诱导而来的、通过体外标准化的模拟胚胎发育获得的β细胞。这些细胞分泌大量的胰岛素和C-肽，并且大量表达胰岛素/C-肽、 MAFA、NKX6.1、PDX1、UCN3等蛋白质。完整的试剂盒可以作为理想的实验工具，应用于研究β细胞功能、糖尿病/疾病模型构建、以及胰岛素分泌和调节的化合物筛选。
Cellartis®β细胞是由我们的人iPS细胞系（ChiPSC12；ChiPSC22）分化而来，制备成单细胞悬液，冻存在管中。该试剂盒含有β细胞，包被涂层，基础培养基，以及补充剂。目前提供两种试剂盒供选择，每个试剂盒中的β细胞都来自健康人供体，其中Cellartis hiPS Beta Cells (from ChiPSC22) Kit（Code No. Y10106）的供体细胞是对糖尿病易感的HLA(human leukocyte antigen人类白细胞抗原)类型，HLA-A*02:01。
 
■ 产品特点
· Beta cells from ChiPSC12 and ChiPSC22 originate from healthy human donors
· Beta cells from ChiPSC22 originate from a donor with a diabetes-susceptible HLA type, HLA-A*02:01 (Marron et al. 2002)
· Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type
· Cells show protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3
· Cells demonstrate insulin and C-peptide secretion
· Cells were derived with a protocol that mimics embryonic development
· Cells provide reproducible and biologically relevant data
 
■ 更多信息
Donor information
The tissues used for the reprogramming of somatic cells into hiPSCs are from donors who have signed informed consent which outlines in detail the purpose of the donation and the procedure for processing of the donated tissue. In order to protect the privacy and the confidentiality of the donors, all identifiers associated with the donors have been removed. The donor consent was obtained for commercial use. The donation did not result in any financial gain for the donors.
· ChiPSC12 was sourced from skin fibroblasts from a healthy 24-year-old European/North African male volunteer (77 kg/177 cm).
· ChiPSC22 was sourced from skin fibroblasts from a healthy 32-year-old European/North African male volunteer (74 kg/179 cm) with a diabetes-susceptible HLA type, HLA-A*02:01.
 
HLA typification data for each line is shown below.
胰腺β细胞Human Stem Cell Derived Beta Cells
 
■ 产品应用
· Disease modeling for diabetes
· Compound screening for insulin secretion and regulation
· Beta-cell functionality
· GSIS (glucose-stimulated insulin secretion) analysis
· Incretin response studies
· Autoimmune beta-cell destruction
· Pancreatitis and beta-cell function
· Transdifferentiation
 
■ 产品组成
· 1 vial (4.8 x 106 viable cells) of Cellartis hiPS Beta Cells (from ChiPSC12) or ChiPSC22
· 11 vials (360 μl/vial) of Cellartis Beta Cell Supplement
· 1 tube (4 ml) of Cellartis Beta Cell Coating
· 1 bottle (160 ml) of Cellartis Beta Cell Basal Medium
· 1 bottle (30 ml) of Cellartis Beta Cell Basal Medium 2
 
 
[Cellartis hiPS Beta Cells (from ChiPSC12) 的胰岛素和 C-肽免疫荧光染色]
胰腺β细胞Human Stem Cell Derived Beta Cells
解冻后两周的β细胞进行成熟标志胰岛素(A图) 和 C-肽 (B图)免疫荧光染色。合并数据显示了胰岛素和 C-肽共表达细胞 (C图)。细胞核使用DAPI 染色(D图)。
  （图片来源于Takara Bio USA, Inc.）
 
■ 技术资料
胰腺β细胞Human Stem Cell Derived Beta Cells
Cellartis human iPS cell-derived beta cells for diabetes drug discovery and disease modeling
Studying diabetes and metabolic disorders using hiPSC-derived beta cells.
 
■ 参考文献
Marron, M. et al. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Nat Acad Sci U. S. A. 99, 13753–8 (2002).
 
 
 
■ 产品详情请点击：胰腺β细胞Human Stem Cell Derived Beta Cells
 
 
 

页面更新：2024-02-29 10:53:42

CHIR-02/10/50-CHIR99021，CHIR-02/10/50-小分子化合物

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产品名称：CHIR99021
产品编号：CHIR-02/10/50
品牌；StemRD
厂家：StemRD
产品规格：2/10/50mg
储存条件：-80℃
到货时间：2-5天

产品信息

Wnt信号通路是一个复杂的蛋白质作用网络，其功能zui常见于胚胎发育和癌症，但也参与成年动物的正常生理过程。Wnt信号通路包括许多可调控Wnt信号分子合成的蛋白质，它们与靶细胞上的受体相互作用，而靶细胞的生理反应则来源于细胞和胞外Wnt配体的相互作用。

CHIR99021 (CT99021)
is a glycogen synthase kinase 3β (GSK3β) inhibitor with IC50 less than 10 nM. Compared to other GSK-3 inhibitors, CHIR-99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) with 350-fold selectivity toward GSK-3β compared to CDKs .
Ref: Tighe A et al. BMC Cell Biol. 2007 Aug 14;8:3
Purity:
Greater than 99% as determined by LC/MS analysis. LC/MS and/or NMR data available upon request.
Biological Activity:
In a cell-based assay measuring the activation of the TCF reporter gene, this compound gives EC50 of 50 nM.

磺基-CY3 羧酸

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磺基-CY3 羧酸

货号：
IC0770

品牌：
Jinpan

磺基-CY3 羧酸

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产品简介
MDL MFCD28978247
别名 Sulfo-Cyanine3
CAS 146368-13-0
分子式 C31H38N2O8S2
分子量 630.77
纯度 ≥98%
单位 瓶
SMILES CC(/C(N1CCCCCC(O)=O)=CC=CC2=[N+](CC)C(C=CC(S([O-])(=O)=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4
规格 5mg
是一种荧光标记染料（λex= 554，λem= 568）

Tergitol-7琼脂

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Tergitol-7琼脂

货号：
LA2370

品牌：
Jinpan

产品简介
储存条件 常温干燥
单位 瓶
规格 250g

用于大肠菌群的鉴定（NMKL），每100ml培养基中添加1支致泻大肠埃希氏菌套装生化鉴定管，包含蛋白胨水、半固体琼脂、氰化钾、氰化钾对照、赖氨酸、氨基酸对照、尿素琼脂、氧化酶等。

配制方法:
称取本品56.2g于1L蒸馏水或去离子水中（可按比例增加或减少配制量）,加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,冷却至50℃左右时，每100mL培养基中加入1支致泻大肠埃希氏菌套装生化鉴定管，混匀倒入无菌平皿，备用。
配方： g/L
胰酪蛋白胨                10.0g
酵母浸粉                  6.0g
牛肉浸粉                  5.0g
乳糖                      20.0g
溴麝香草酚蓝              0.05g
Tergitol-7                0.1g
琼脂                      15.0g          
pH7.0±0.2(25℃)