BioXCell热销产品–RecombiMAb anti-mouse CD40
BioXCell热销产品–RecombiMAb anti-mouse CD40
产品描述:
FGK4.5-CP133单克隆抗体是原始FGK4.5单克隆抗体的重组嵌合型抗体。可变结构域序列与原始FGK4.5克隆号相同,但是恒定区序列已经从大鼠IgG2a变为小鼠IgG2a。FGK4.5-CP133抗体像原始大鼠IgG2a抗体一样,不包含Fc突变。
FGK4.5-CP133单克隆抗体能与小鼠CD40(也称为Bp50)反应。CD40是一种48 kDa的I型跨膜糖蛋白,属于肿瘤坏死因子受体(TNFR)超家族。CD40广泛表达于抗原呈递细胞(APCs),如树突状细胞、B细胞、巨噬细胞和单核细胞,以及非免疫内皮细胞、基底上皮细胞和一系列肿瘤细胞上。CD40与其配体CD154结合后,作为共刺激分子激活B细胞、树突状细胞、单核细胞和其他APCs。CD40在B细胞活化、分化、增殖和免疫球蛋白同种型转换以及树突状细胞成熟中起作用。激动性CD40单克隆抗体已被证明能激活APCs并促进抗肿瘤T细胞反应。FGK4.5单克隆抗体是一种激动性抗体,已被证明能激活表达CD40的APC。FGK4.5单克隆抗体也可用于体外和体内抑制CD40/CD154相互作用。

产品详情:
产品名称
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RecombiMAb anti-mouse CD40
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产品货号
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CP133
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产品规格
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1/5/25/50/100mg
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反应种属
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Mouse
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克隆号
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FGK4.5-CP133
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同种型
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Mouse IgG2a(switched from rat IgG2a)
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免疫原
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Recombinant mouse CD40 fusion protein
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实验应用
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in vivo CD40 activation* in vitro B cell stimulation/activation* *Reported for the original rat IgG2a FGK4.5 antibody
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产品形式
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PBS, pH 7.0,Contains no stabilizers or preservatives
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纯度
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>95%, Determined by SDS-PAGE
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聚合
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<5%, Determined by SEC
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无菌处理
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0.2 µm filtration
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纯化方式
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Protein G
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分子量
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150 kDa
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小鼠病原检测
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Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
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保存条件
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抗体原液保存在4°C,不能冷冻保存。
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推荐同型对照
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InVivoPlus mouse IgG2a isotype control, unknown specificity(货号BP0085)
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推荐抗体稀释液
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InVivoPure pH 7.0 Dilution Buffer(货号IP0070)
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该产品自上市已被多篇SCI文献引用,品质有保证,以下是部分已发表的文献引用:
应用
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文章
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体内CD40激活
(in vivo CD40 activation)
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1. Bauche, D., et al. (2018). ‘LAG3(+) Regulatory T Cells Restrain Interleukin-23-
Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid
Cell-Driven Colitis’ Immunity 49(2): 342-352 e345.
2. Carmi, Y., et al. (2015). ‘Allogeneic IgG combined with dendritic cell stimuli induce
antitumour T-cell immunity’ Nature 521(7550): 99-104.
3. Conde, P., et al. (2015). ‘DC-SIGN(+) Macrophages Control the Induction of
Transplantation Tolerance’ Immunity 42(6): 1143-1158.
4. Bartkowiak, T., et al. (2015). ‘Unique potential of 4-1BB agonist antibody to promote
durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine’ Proc
Natl Acad Sci U S A 112(38): E5290-5299.
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体外B细胞刺激/激活
(in vitro B cell stimulation/activation)
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1. Xu, H., et al. (2015). ‘Regulation of bifurcating B cell trajectories by mutual antagonism
between transcription factors IRF4 and IRF8′ Nat Immunol .
2. Muppidi, J. R., et al. (2014). ‘Loss of signalling via Galpha13 in germinal centre
B-cell-derived lymphoma’ Nature 516(7530): 254-258.
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更多产品详情请咨询 BioXCell 中国代理——上海金畔生物