Mouse anti-TIMP2 Monoclonal Antibody(1554CT494.262.47)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
TIMP2抗体;Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2; TIMP2
|
| 宿主 |
Mouse
|
| 特异性 |
This TIMP2 antibody is generated from a mouse immunized with arecombinant protein of human TIMP2.
|
| 反应种属 |
Human
|
| 应用 |
IF~~1:25
FC~~1:25 WB~~1:2000 IHC-P~~1:25 |
| 分子量 |
Predicted molecular weight: 24kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1554CT494.262.47
|
| 同种型 |
IgG1,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cancer;Cardiovascular;Cell Biology;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
|
| 细胞定位 |
Secreted.
|
| UniProt |
P16035
|
| 参考文献 | |
| 参考文献 |
Stetler-Stevenson W.G.,et al.J. Biol. Chem. 265:13933-13938(1990).
Boone T.C.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:2800-2804(1990). Hammani K.,et al.J. Biol. Chem. 271:25498-25505(1996). Malik K.,et al.Submitted (AUG-1990) to the EMBL/GenBank/DDBJ databases. Stetler-Stevenson W.G.,et al.J. Biol. Chem. 264:17374-17378(1989). |
实验结果图
Anti-TIMP2 Antibody at 1:500 dilution + SW480 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TIMP2 Antibody at 1:2000 dilution + HT-1080 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.