LT07-705-Lonza LT07-705 MycoAlert™ PLUS 支原体检测试剂盒(新一代),50Tests-支原体检测试剂盒

MycoAlert™ PLUS Mycoplasma Detection Kit
货号:LT07-705
厂家:美国Lonza公司
品牌:Lonza
规格:50T/盒
产品应用:支原体检测试剂盒
保存温度:2℃-8℃
会员优惠价格: 请
货期:2~5天

产品英文名称:MycoAlert™ PLUS Mycoplasma Detection Kit
产品中文名称:MycoAlert™ PLUS 支原体检测试剂盒

MycoAlert™ PLUS支原体检测试剂盒 产品介绍:
MycoAlert™ PLUS Assay是一种精选生物化学检测方法,用于检测细胞培养过程中的支原体污染问题。支原体是一种能够自我复制的zui小的原核微生物,和其他细菌不同的是它们没有细胞壁,所以细胞形态不固定,可以有多种变化,可以在培养基上形成极小的菌落。支原体是寄生生物,但同时也具有腐生功能。细胞培养过程中的污染支原体会使细胞生长变慢,影响细胞活性变化。新一代MycoAlert™ PLUS Assay可以检测出所有6种支原体属,多达180种支原体。与*代MycoAlert™支原体检测试剂盒相比有更高的灵敏度和准确性。检测样品中的活性支原体裂解后,释放出的酶与MycoAlert™ PLUS Substrate反应,催化ADP转化为ATP。加入MycoAlert™ PLUS Reagent试剂后在荧光素酶作用下ATP产生光信号(ATP+ Luciferin + O2 →Oxyluciferin + AMP + PPi + CO2 + LIGHT)。光信号可用光度计读出,根据第二次读数(Read B)与*次读数(Read A)比率可是确定是否存在支原体污染。Read B/Read A比率<1, 支原体阴性,无支原体;1-1.2,不确定是否存在支原体,需重新检测;>1.2,支原体阳性,存在支原体污染。

产品优点:
» 生物发光技术,无需DNA提取物
» 样品检测只需两步移液操作,结果分析简单
» 可以和低灵敏性的板光度计或多功能读数器配用
» 可用于检测未启用培养基,培养基补充物或水溶液
» 快速方便,标准程序20分钟就可出结果
» 通用性强,可检测所有常见的支原体污染(不含脲原体属)

适合检测样品:
– 悬浮细胞上清液
– 未启用的培养基
– 10%-20% FCS or FBS
– L-谷氨酰胺
– 青霉素-链霉素混合物
– 中性胰蛋白酶(1:2稀释)
– 庆大霉素/两性霉素B

MycoAlert™ PLUS Mycoplasma Detection Kit:
Mycoplasma are the smallest and simplest prokaryotes. Mycoplasma depend on their hosts for
many nutrients due to their limited biosynthetic capabilities. They have long been recognized as
common contaminants of cells in continuous culture, but their presence may go undetected for months. As the mycoplasma competes with the cells for the nutrients in culture media, one of the first signs is a reduction in the rate of cell proliferation and slight changes in cellular responses, including gene expression.
Mycoplasma detection in cell cultures has, until now, been a long, drawn out process with difficult-tointerpret results. The MycoAlert™ PLUS Kit is intended for the quick and convenient detection of viable mycoplasma in cell cultures. The speed and convenience of the MycoAlert™ PLUS Kit allows for the routine testing of cells in culture and commonly used constituents of complete media.
To allow for the early detection of mycoplasma contamination, Lonza recommends testing at every cell passage. Frequent testing such as this will indicate when a cell line becomes infected, allowing prompt remedial action to be taken. The MycoAlert™ PLUS Assay can also be extended to incoming cell lines and the commonly used constituents of complete media.

订购信息:
• LT07-701 MycoAlert™ PLUS Mycoplasma Detection Kit, 10Tests
• LT07-703 MycoAlert™ PLUS Mycoplasma Detection Kit, 30Tests
• LT07-705 MycoAlert™ PLUS Mycoplasma Detection Kit, 50Tests
• LT07-710 MycoAlert™ PLUS Mycoplasma Detection Kit, 100Tests
• LT07-518 MycoAlert™ Assay Control Sets, 10Tests
• LT27-292 MycoAlert™ Assay Buffer, 20ml

Amersco-K249-1L-A.C.E.测序缓冲液(10X)-amresco试剂

【简单介绍】

A.C.E.测序缓冲液(10X),英文名称:A.C.E.SEQUENCING BUFFER(10X RUNNING BUFFER),Grade:BIOTECHNOLOGY GRADE,包装规格:1L,CAS:N/A

【详细说明】

A.C.E.测序缓冲液(10X),英文名称:A.C.E.SEQUENCING BUFFER(10X RUNNING BUFFER),Grade:BIOTECHNOLOGY GRADE,包装规格:1L,CAS:N/A

K249

A.C.E.™ SEQUENCING BUFFER, 10X CONCENTRATE

Grade:BIOTECHNOLOGY GRADE

CAS Number: N/ASynonym(s): None

Molecular Weight: N/AMolecular Formula: N/A

Storage Condition: ROOM TEMPERATURE

CodeSizePriceQuantity

K249-100ML

K249-1L

1004-070WHATMAN4号快速定性滤纸直径70mm货号1004-070

【简单介绍】

WHATMAN4号快速定性滤纸直径70mm货号1004-070,Whatman定性滤纸用于定性分析技术中鉴定物质的性质。极快的过滤速度,非常好的截留粗颗粒和胶状沉淀物例如氢氧化铁和氢氧化铝。非常合适作为快速过滤在日常分析中进行生物或者有机浸出物的快速过滤。在高流速空气污染监测,如果不需要截留细小颗粒,也可使用。

【详细说明】

WHATMAN4号快速定性滤纸直径70mm货号1004-070

Whatman定性滤纸用于定性分析技术中鉴定物质的性质。

Grade 4:20-25μm,极快的过滤速度,非常好的截留粗颗粒和胶状沉淀物例如氢氧化铁和氢氧化铝。非常合适作为快速过滤在日常分析中进行生物或者有机浸出物的快速过滤。在高流速空气污染监测,如果不需要截留细小颗粒,也可使用。

WHATMAN4号快速定性滤纸直径70mm货号1004-070订购信息:

1004-070WHATMAN4号快速定性滤纸直径70mm货号1004-070

96孔吸头,200μL,透明,过滤,无菌MaxymumRecovery

96孔吸头,200μL,透明,过滤,无菌MaxymumRecovery

货号:
VTF-205-L-R-S

品牌:
Axygen

产品简介
单位
英文名称 Automation Tips for Agilent /Velocity11 VPrep and Bravo, 200μL, Clear, Filtered, Sterile, Maxymum Recovery
规格 96支/盒,10盒/大盒,5大盒/箱

Amersco-0131-2.5KG-醋酸镁-amresco试剂

【简单介绍】

醋酸镁,英文名称:MAGNESIUM ACETATE, TETRAHYDRATE,等级:分析级,包装规格:2.5KG,CAS:16674-78-5

【详细说明】

醋酸镁,英文名称:MAGNESIUM ACETATE, TETRAHYDRATE,等级:分析级,包装规格:2.5KG,CAS:16674-78-5

0131
MAGNESIUM ACETATE TETRAHYDRATE
Grade:ANALYTICAL GRADE
CAS Number: 16674-78-5Synonym(s): None
Molecular Weight: 214.45Molecular Formula: (CH3COO)2Mg.4H2O
Storage Condition: ROOM TEMPERATURE

酶结合物稳定剂

酶结合物稳定剂

StabilZyme产品系列专门用于稳定蛋白结合物,能保存蛋白质的立体结构、维持多肽链的折叠状态。StabilZyme AP和StabilZyme HRP分别用于保护碱性磷酸酶标记和辣根过氧化物酶标记的抗体。StabilZyme SELECT推荐用于稳定竞争法检测分析中HRP-半抗原结合物,也可用于稳定血清及其它溶液中的蛋白。

产品信息

 

产品编号  产品名称  规格  缓冲液  PH值  保存条件  有效期(4℃)
 SA01-1000  StabilZyme® AP Conjugate Stabilizer  1000ml  MES  5.5 -6.5  冷藏或室温  2.5年
 SA01-2000  StabilZyme® AP Conjugate Stabilizer  2000ml  MES  5.5 -6.5  冷藏或室温  2.5年
 SZ02-1000  StabilZyme® HRP Conjugate Stabilizer  1000ml  MOPS  6.2-6.7  冷藏或室温  2.5年
 SZ02-2000  StabilZyme® HRP Conjugate Stabilizer  2000ml  MOPS  6.2-6.7  冷藏或室温  2.5年
 SZ03-1000  StabilZyme SELECT® Stabilizer  1000ml  None  6.5-7.5  冷藏或室温  2.5年
 SZ03-2000  StabilZyme SELECT® Stabilizer  2000ml  None  6.5-7.5  冷藏或室温  2.5年
 SZ04-1000  StabilZyme® NOBLE Stabilizer (BSA-Free)  1000ml  MOPS  6.0-7.0  冷藏或室温  2.5年

酶结合物稳定剂

                                                    
图1.SA01                                                                                                           图4. SZ04

                                                    
 图3.SZ03                                                                                                           图2. SZ02

稳定性研究
 StabilZyme AP稳定剂、StabilZyme HRP稳定剂、StabilZyme SELECT稳定剂、StabilZyme NOBLE稳定剂都经过了酶活和免疫活性
 测试(见图1、图2、图3、图4)
 StabilZyme AP稳定剂使用单克隆IgG-AP结合物,和一般缓冲液的AP结合物比较,室温储存24个月后,单克隆IgG-AP结合物的活性仍
 保持70%
 StabilZyme HRP稳定剂使用单克隆IgG-HRP结合物,和一般缓冲液的HRP结合物比较,室温储存24个月后,单克隆IgG-HRP结合物还
 有大于90%的活性
 StabilZyme SELECT稳定剂使用单克隆IgG-HRP结合物,和一般缓冲液的HRP结合物比较,室温储存24个月后,单克隆IgG-HRP结合
 物还有75%的活性
 为了证明StabilZyme NOBLE稳定剂的稳定效率,把四种IgG-HRP结合物分别储存在StabilZyme NOBLE稳定剂中。HRP结合物包括:
 兔抗鼠IgG-HRP、鼠抗兔IgG-HRP、羊抗兔IgG-HRP、鸡(IgY)抗鼠 IgG-HRP。把储存在4℃的对照抗体结合物和储存在37℃稳定剂中的
 IgG-HRP结合物进行比较,结果显示37℃保存30天后四种HRP结合物还有至少80%的活性

Adenosine 腺苷

Adenosine 腺苷

货号:
IA0100

品牌:
Jinpan

Adenosine  腺苷

暂无详情

Adenosine  腺苷

来源文献:Zhou N , et al . Food Funct. 2018 Sep 19;9(9):4989-4997.

Adenosine  腺苷

来源文献:Zhou N , et al . Food Funct. 2018 Sep 19;9(9):4989-4997.

Adenosine  腺苷

来源文献:Zhou N , et al . Food Funct. 2018 Sep 19;9(9):4989-4997.

Adenosine  腺苷

来源文献:Zhou N , et al . Food Funct. 2018 Sep 19;9(9):4989-4997.
产品简介
MDL MFCD00005752
EC EINECS 200-389-9
别名 腺素苷; ?腺嘌呤核苷
CAS 58-61-7
分子式 C10H13N5O4
分子量 267.24
纯度 HPLC≥98%
单位
生物活性 Adenosine (Adenine riboside), a ubiquitous endogenous autacoid, acts through the enrollment of four G protein-coupled receptors: A1, A2A, A2B, and A3. Adenosine affects almost all aspects of cellular physiology, including neuronal activity, vascular function, platelet aggregation, and blood cell regulation[1-2].
In Vitro Adenosine (Adenine riboside) acts on four G-protein coupled receptors: two of them, A1 and A3, are primarily coupled to Gi family G proteins; and two of them, A2A and A2B, are mostly coupled to Gs like G proteins. These receptors are antagonized by xanthines including caffeine. Via these receptors it affects many cells and organs, usually having a cytoprotective function[2].Adenosine is an extracellular signaling molecule that is generated from its precursor molecules 5’-adenosine triphosphate (ATP) and 5’-adenosine monophosphate (AMP)[3].Adenosine is a common metabolite of ATP, which exhibits cytotoxic effects at high concentrations.Adenosine (1.0-4.0 mM; 12-24 hours) inhibits cell viability and triggers ER stress in HepG2 cells.Adenosine induces apoptosis in a variety of cancer cells. Adenosine (2.0 mM; 12-24 hours) induces autophagy in HepG2 cells. In HepG2 cell lines, Adenosine -induced AMPK/mTOR pathway activation partially blocked ER stress and decreased apoptotic cell death[4].
SMILES NC1=C2C(N([C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3)C=N2)=NC=N1
靶点 DNA/RNA Synthesis
数据来源文献 [1]. Fredholm BB. Adenosine, an endogenous distress signal, modulates tissue damage and repair. Cell Death Differ. 2007;14(7):1315-1323.

[2]. Borea PA, Gessi S, Merighi S, Vincenzi F, Varani K. Pharmacology of Adenosine Receptors: The State of the Art. Physiol Rev. 2018;98(3):1591-1625.

[3]. Eltzschig HK. Adenosine: an old drug newly discovered. Anesthesiology. 2009;111(4):904-915.

[4]. Zhou XT, et al. Inhibition of autophagy enhances adenosine induced apoptosis in human hepatoblastoma HepG2 cells. Oncol Rep. 2019;41(2):829-838.

规格 10mg 50mg 10mM*1mL (in DMSO)

腺苷是一种遍布人体细胞的内源性核苷,是用于合成三磷酸腺苷(ATP)、腺嘌呤、腺苷酸、阿糖腺苷的重要中间体。可直接进入心肌经磷酸化生成腺苷酸。

使用本产品的应用案例(仅供参考

In Vivo

Rat(Adenosine 50mg/kg,口服,2次/天,3天)

Seventy rats were randomly divided into 7 groups (female and male in equal numbers for each group): control group(8, C), SCM model group (12, SCM), AI treated group (10, AI,200 mg kg−1), CY extract treated group (10, CY, 1630 mg kg−1),Ade treated group (10, Ade, 50 mg kg−1)Arb treated group (10,Arb, 50 mg kg−1) and All treated group (10, All, 50 mg kg−1).The SCM model was induced by subcutaneous injection of LPS at a dose of 10 mg kg−1. The drugs were orally administered twice a day for 3 days, respectively. The C and SCM groups were orally administered with the same volume of distilled water in the meantime. All rats were sacrificed after the detection of the cardiac function and the blood sample was obtained from the abdominal aorta.

来源文献:Zhou N , Zeng MN , Li K , Yang YY , Bai ZY , Zheng XK , Feng WS . An integrated metabolomic strategy for the characterization of the effects of Chinese yam and its three active components on septic cardiomyopathy. Food Funct. 2018 Sep 19;9(9):4989-4997. doi: 10.1039/c8fo00688a. PMID: 30187904.