Fluorescent Probes（荧光探针）—cayman品牌推荐
Cayman拥有500多种荧光探针，帮助您提升研究中所需的多功能性、灵敏度和定量能力。这一系列探针能够检测细胞内活动、蛋白质相互作用、一系列酶活性和许多其他重要目标蛋白。为实验选择最合适的荧光染料请参考以下cayman荧光探针表格。
检测细胞活力和细胞增殖的探针
| Item No. |
Product Name |
Detects |
Cell Permeable? |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 11397 |
7-Aminoactinomycin D |
DNA; non-viable cells |
× |
488,546,578 |
650 |
| 14948 |
Calcein AM* |
cell viability |
√ |
494 |
517 |
| 20637 |
Calcein Blue AM* |
cell viability |
√ |
360 |
445 |
| 20632 |
Calcein Red™ AM* |
cell viability |
√ |
560 |
574 |
| 20639 |
Calcein UltraBlue™ AM* |
cell viability (higher photostability and stronger fluorescence intensity atphysiological pH than Calcein Blue) |
√ |
360 |
445 |
| 14456 |
CFDA-SE |
cell proliferation |
√ |
491 |
518 |
| 20698 |
CytoTrace™ Red CMTPX |
cell division; cell tracking |
√ |
577 |
602 |
| 35701 |
Fluorescein diacetate |
cell viability |
√ |
490 |
520 |
| 19583 |
Green CMFDA |
cell division; cell tracking |
√ |
492 |
517 |
| 15003 |
JC-1 |
mitochondrial integrity; apoptosis |
√ |
485(monomer)
520-570(aggregate) |
535(monomer)
570-610(aggregate) |
|
| 14289 |
Propidium Iodide |
DNA; RNA; non-viable cells |
× |
488-535 |
617 |
| 14322 |
Resazurin (sodium salt) |
enzyme activity; cell viability |
√ |
570 |
580 |
| 21426 |
Tetramethylrhodamine ethyl ester (perchlorate) |
mitochondrial function; cell viability |
√ |
550 |
575 |
*Also available: membrane-impermeable non-AM forms
View a complete list of cell viability fluorescent probes at www.caymanchem.com
检测核苷酸的探针
| Item No. |
Product Name |
Detects |
Cell Permeable? |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 14338 |
Acridine Orange |
Nucleic acids; pH |
√ |
502(dsDNA)
460(ssDNA andRNA)
475 (low pH) |
525(dsDNA)
650(ssDNA and RNA)
590(low pH) |
| 22439 |
9-Amino-6-chloro-2- methoxyacridine |
DNA and pH gradients; poly(dA-dT) sequences of DNA |
√ |
411 |
475 |
| 11315 |
Chromomycin A3 |
DNA (two or more contiguous GC base pairs) |
× |
445 |
575 |
| 14285 |
DAPI (hydrochloride) |
DNA (AT-rich sequences) |
× |
358 |
461 |
| 15547 |
Hoechst 33342 (hydrochloride) |
DNA (AT-rich sequences); nuclear marker |
√ |
350 |
461 |
| 14488 |
Pyronin Y |
dsRNA |
√ |
540-550 |
560-580 |
View a complete list of DNA fluorescent probes at www.caymanchem.com
检测氧化应激和活性反应组分的探针
| Item No. |
Product Name |
Detects |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 10157 |
APF |
hydroxyl radical; hypochlorite ion; certain peroxidase intermediates |
490 |
515 |
| 27086 |
C11 BODIPY 581/591 |
lipid oxidation |
581/500(oxidized) |
591/510(oxidized) |
| 14051 |
Coumarin Boronic Acid |
peroxynitrite; hypochlorous acid; hydrogen peroxide |
332 |
470 |
| 19111 |
Coumarin hydrazine |
reactive carbonyl groups |
365 |
430/550
(upon reaction with carbonyls) |
| 85165 |
DAF-2 diacetate |
nitric oxide |
485 |
538 |
| 18767 |
DAF-FM diacetate |
nitric oxide |
495 |
515 |
| 85155 |
2,7-Dichlorodihydrofluorescein diacetate |
peroxynitrite formation |
502 |
523 |
| 12013 |
Dihydroethidium |
superoxide |
490 |
590 |
| 85100 |
Dihydrorhodamine 123 |
peroxynitrite formation |
500 |
536 |
| 62237 |
DPPP |
hydroperoxides; low-density lipoprotein; cellular oxidation |
351 |
380 |
| 34564 |
7-Fluoro-2,1,3-benzoxadiazole- 4-sulfonate (ammonium salt) |
thiols |
380 |
515 |
| 14872 |
Lucigenin |
superoxide; chloride |
455 |
505 |
| 16803 |
Luminol |
peroxidation; iron; gold; cyanide |
355 |
411 |
| 17097 |
Monobromobimane |
reactive sulfur; thiol groups |
398 |
490 |
| 10005983 |
Pentafluorobenzenesulfonyl fluorescein |
hydrogen peroxide |
465-505 |
505-555 |
| 16929 |
WSP-5 |
hydrogen sulfide |
502 |
525 |
View a complete list of fluorescent probes for oxidative stress and reactive species detection at www.caymanchem.com
检测细胞骨架蛋白的探针
| Item No. |
Product Name |
Detects |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 21016 |
9-(2,2-Dicyanovinyl)julolidine |
tubulin and other cytoskeletal proteins |
450 |
480(low viscosity solvents)
505(high viscosity solvents) |
| 20546 |
Phalloidin-California Red™ Conjugate |
F-actin |
583 |
605 |
| 20478 |
Phalloidin-Fluorescein Conjugate |
F-actin |
492 |
518 |
| 20547 |
Phalloidin-iFluor™ 350 Conjugate |
F-actin |
353 |
442 |
| 20548 |
Phalloidin-iFluor™ 405 Conjugate |
F-actin |
400 |
421 |
| 20549 |
Phalloidin-iFluor™ 488 Conjugate |
F-actin |
493 |
517 |
| 20552 |
Phalloidin-iFluor™ 555 Conjugate |
F-actin |
556 |
574 |
| 20553 |
Phalloidin-iFluor™ 594 Conjugate |
F-actin |
590 |
618 |
| 20554 |
Phalloidin-iFluor™ 633 Conjugate |
F-actin |
634 |
649 |
| 20555 |
Phalloidin-iFluor™ 647 Conjugate |
F-actin |
650 |
665 |
| 20545 |
Phalloidin-Tetramethylrhodamine Conjugate |
F-actin |
546 |
575 |
View a complete list of cytoskeletal protein fluorescent probes at www.caymanchem.com
 |
 |
|
Actin filaments stained with
Phalloidin-iFluor™ 350 Conjugate |
HeLa cells stained with
Phalloidin-iFluor™ 488 Conjugate |
HeLa cells labeled with
Phalloidin-iFluor™ 594 Conjugate |
蛋白酶底物探针
| Item No. |
Product Name |
Detects |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 9002021 |
Abz-Val-Ala-Asp-Nva-Arg-Asp- Arg-Gln-EDDnp (trifluoroacetate salt) |
human proteinase 3 activity |
320 |
420 |
| 26640 |
Ac-ANW-AMC |
β5i/LMP7 subunit of the 20S immunoproteasome activity |
340-360 |
440-460 |
| 14986 |
Ac-DEVD-AMC |
caspase-3 activity |
340-360 |
440-460 |
| 17051 |
Ac-LEHD-AFC (trifluoroacetate salt) |
caspase-4, -5, and -9 activity |
400 |
505 |
| 14991 |
Ac-LETD-AFC |
caspase-8 activity |
400 |
505 |
| 14988 |
Ac-VDVAD-AFC |
caspase-2 activity |
400 |
505 |
| 14992 |
Ac-WEHD-AFC (trifluoroacetate salt) |
caspase-1, -4, and -5 activity |
400 |
505 |
| 17591 |
Ac-YVAD-AFC |
caspase-1 and -4 activity |
400 |
505 |
| 27580 |
Gly-Arg-AMC (hydrochloride) |
cathepsin C activity |
340-360 |
440-460 |
| 14907 |
MeOSuc-Ala-Ala-Pro-Val-AMC |
human leukocyte and porcine pancreatic elastase activity |
355-380 |
440-460 |
| 10008119 |
Suc-Leu-Leu-Val-Tyr-AMC |
20S proteasome, chymotrypsin-like proteases, and calpain activity |
360 |
460 |
| 11675 |
(Z-Ala-Ala-Ala-Ala)2Rh110 |
elastase activity |
485 |
525 |
| 10008117 |
Z-LLE-AMC |
caspase-like activity of the 26S proteasome or 20S proteolytic core |
340-360 |
440-460 |
View a complete list of protease activity fluorescent probes at www.caymanchem.com
其他酶底物探针
| Item No. |
Product Name |
Detects |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 62910 |
7-hydroxycoumarinyl Arachidonate |
cPLA2 activity |
335 |
450 |
| 25583 |
C12FDG |
β-galactosidase activity |
485 |
530 |
| 35500 |
DiFMUP |
phosphatase activity |
358 |
455 |
| 16551 |
4-Methylumbelliferyl-α-D- Galactopyranoside |
α-galactosidase activity |
330(pH 4.6);
370 (pH 7.4);
385(pH 10.4) |
445-454 |
| 20948 |
4-Methylumbelliferyl-β-D- Glucopyranoside |
β-glucosidase and β-glucocerebrosidase activity |
330(pH 4.6);
370 (pH 7.4);
385(pH 10.4) |
445-454 |
| 19543 |
4-Methylumbelliferyl-α-L- Iduronide (free acid) |
α-L-iduronidase activity |
320(pH 1.97-6.72);
360(pH 7.12-10.3) |
445-455
(increases as pH decreases) |
| 19524 |
4-Methylumbelliferyl 6-thio- Palmitate-β-D-Glucopyranoside |
palmitoyl-protein thioesterase (PPT/CLN1) activity |
320(pH 1.97-6.72);
360(pH 7.12-10.3) |
445-455
(increases as pH decreases) |
View a complete list of enzyme substrate fluorescent probes at www.caymanchem.com
检测蛋白交互作用探针
| Item No. |
Product Name |
Detects |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 20672 |
3-BODIPY-propanoylaminocaproic Acid N-hydroxysuccinimide ester |
proteins immobilized on PVDF membranes |
300-360,504 |
511 |
| 20704 |
FlAsH-EDT2 |
proteins with a tetracysteine sequence |
508 |
528 |
| 19767 |
ReAsH-EDT2 |
proteins with a tetracysteine sequence |
593 |
608 |
View a complete list of probes to detect protein interactions at www.caymanchem.com
检测钙离子探针
| Item No. |
Product Name |
Detects |
Cell
Permeable?
|
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 20424 |
BTC AM* |
intracellular calcium (low affinity) |
√ |
401(Ca2+-bound)/480 |
529 |
| 35758 |
Fluo-2 AM* |
intracellular calcium |
√ |
490 |
515 |
| 14960 |
Fluo-3 AM* |
intracellular calcium |
√ |
488 |
526 |
| 31143 |
Fluo-4 AM* |
intracellular calcium |
√ |
490 |
520 |
| 14591 |
Fura-2 AM* |
intracellular calcium |
√ |
380 (low [Ca2+])
340 (high [Ca2+]) |
510 |
| 34993 |
Fura-2 Leakage Resistant AM* |
intracellular calcium |
√ |
340 (Ca2+-bound)
380 (Ca2+-free) |
505 |
| 20416 |
Fura-FF AM* |
intracellular calcium (low affinity) |
√ |
365 (no Ca2+)
339 (high [Ca2+]) |
514(no Ca2+)
507(high [Ca2+]) |
| 34985 |
ICR-1 AM* |
intracellular calcium |
√ |
580 |
660 |
| 20417 |
Indo-1 AM* |
intracellular calcium |
√ |
349-364 |
475-485(no Ca2+)
400-410(Ca2+-bound) |
| 20442 |
Rhod-5N AM* |
intracellular calcium (low affinity) |
√ |
551 |
576 |
| 20444 |
Rhod-FF AM* |
intracellular calcium (low affinity) |
√ |
552 |
580 |
*Also available: membrane-impermeable non-AM forms
View a complete list of calcium fluorescent probes at www.caymanchem.com
检测pH值探针
| Item No. |
Product Name |
Detects |
Cell Permeable? |
Excitation
Maximum (nm)
|
Emission
Maximum (nm)
|
| 17183 |
BCECF |
pH |
× |
440 / 490 |
535 |
| 15922 |
BCECF AM |
intracellular pH |
√ |
440 / 490 |
535 |
详情请咨询 Cayman 中国代理-上海金畔生物
