使用Lenti-X Integration Site Analysis Kit检测重组慢病毒载体整合的确切位点。慢病毒的复制生命周期会将慢病毒RNA基因组的一个DNA拷贝整合到宿主基因组中。所选择的整合位点对转基因的表达和宿主细胞的表现型都有重要影响。Lenti-X Integration Site Analysis Kit使用基因组步移技术来识别这些整合位点,以便对其进行测序和分析。
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Sonic Hedgehog, human产品介绍: Hedgehog基因zui早在果蝇中作为体节极性基因被发现,Hedgehog基因编码一种分泌信号蛋白,突变会使无毛部分变成有毛部分,所以又戏称为“刺猬”基因。哺乳动物发现其同源基因有3个,分别为Sonic Hedgehog(SHh)、Indian Hedgehog(IHh)和Desert Hedgehog(DHh)。SHh 前体蛋白要经过一系列剪切与修饰,首先SHh 前体蛋白在内质网剪切去掉信号肽,,然后经过自我酶解形成19 kDa N 端蛋白和25 kDaC 端蛋白,SHh-N 端蛋白发挥了SHh信号分子的功能,执行SHh信号通路的主要功能。C 端蛋白拥有末端胆固醇转移酶活性,可以将胆固醇分子转移到SHh N 端蛋白的C 端,末端的胆固醇分子可以和细胞膜结合而锚定SHh N 端蛋白,从而有效控制SHh N 端蛋白的作用区域。
» Sonic Hedgehog, human蛋白活性比从原核细胞纯化得到的高10-15倍 » 生物活性在Swiss 3T3细胞通过Gli报告基因检测,The EC50 3 to 10 ng/ml » Sonic Hedgehog, human产品是通过哺乳动物细胞纯化得到,并经过SDS-PAGE 和HPLC双重鉴定纯度大于85%
Sonic Hedgehog, human产品英文简述: The hedgehog (Hh) gene encodes a secreted protein originally identified in Drosophila. The vertebrate homologs of Hh comprise of Sonic (SHh), Desert (DHh) and Indian (IHh). SHh plays an important role in embryonic development and in diseases such as cancer. Human SHh precursor is a 45kDa protein. An autocatalytic reaction yields a 19 kDa N-terminal domain that is biologically active. Because of unique post-translational modifications, SHh produced in mammalian cells is much more biologically active than SHh produced from E. coli and is devoid of artifact associated with the E. coli products commercially available from other manufacturers.
• Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis • Biological Activity: The activity was determined by using a Gli reporter gene assay in Swiss 3T3 cells. The EC50 ranges from 3 to 10 ng/ml • For Research Use Only. Not for Use in Humans