【简单介绍】
|
蛋白酶E,英文名称:PRONASE E,Grade:BIOTECHNOLOGY GRADE,包装规格:1G,CAS:9036-06-0 |
【详细说明】
|
蛋白酶E,英文名称:PRONASE E,Grade:BIOTECHNOLOGY GRADE,包装规格:1G,CAS:9036-06-0 E629 ![]() |
【简单介绍】
【详细说明】
名称:Total Goat Milk Proteins WB kit
货号:F245
厂家:Cygnus公司
品牌:Cygnus
规格:1 Kit
保存温度:2-8℃
货期:2~5天
详情请。
现货供应,欢迎订购: :
F245 Total Goat Milk Proteins WB kit
This kit is intended for use in identifying goat milk proteins by Western Blot. The antibodies used in this kit are the same as those used in the corresponding ELISA kit, Catalog # F240.
相关产品:
Product Catalog # Description
A549 HCP ELISA kit F230 Host cell protein assay, 96 tests
A549 HCP Western Blot Kit F235 Host cell protein assay, 5 mini blots
BHK HCP ELISA Kit F510 Host cell protein assay, 96 tests
Bovine IgG ELISA Kit F070 Bioprocess contaminant assay, 96 tests
Bovine Plasma Protein ELISA Kit F290 Bioprocess contaminant assay, 96 tests
BSA ELISA Kit F030 Bioprocess contaminant assay, 96 tests
Bovine Transferrin ELISA Kit F120 Bioprocess contaminant assay, 96 tests
CHO HCP ELISA Kit F015 Host cell protein assay, 96 tests
CHO HCP Western Blot Kit F060 Host cell protein assay, 5 mini blots
CHO-CM ELISA Kit CM015 Host cell protein assay, 96 tests
CHO-CM Western Blot Kit CM060 Host cell protein assay, 5 mini blots
CHO HCP ELISA Kit, 3G F550 Host cell protein assay, 96 tests
E. coli HCP ELISA Kit F410 Host cell protein assay, 96 tests
E. coli HCP Western Blot Kit F415 Host cell protein assay, 5 mini blots
Goat Casein HCP ELISA Kit F420 Host cell protein assay, 96 tests
Goat IgG ELISA Kit F210 Bioprocess contaminant assay, 96 tests
Total Goat Milk Proteins ELISA kit F240 Host cell protein assay, 96 tests
Total Goat Milk Proteins WB kit F245 Host cell protein assay, 5 mini blots
Human Albumin ELISA Kit F055 Bioprocess contaminant assay, 96 tests
Human IgA ELISA Kit F165 Bioprocess contaminant assay, 96 tests
Human IgG-total ELISA Kit F160 Bioprocess contaminant assay, 96 tests
Human IgM ELISA Kit F170 Bioprocess contaminant assay, 96 tests
HEK 293 HCP ELISA Kit F650 Host cell protein assay, 96 tests
Human Transferrin ELISA Kit F035N Bioprocess contaminant assay, 96 tests
Insect Cell SF9 HCP ELISA Kit F020 Host cell protein assay, 96 tests
Insect Cell SF9 Western Blot Kit F125 Host cell protein assay, 5 mini blots
Insulin ELISA Kit F040 Bioprocess contaminant assay, 96 tests
Lactococcus lactic HCP ELISA Kit F490 Host cell protein assay, 96 tests
Lactococcus lactic HCP WB Kit F495 Host cell protein assay, 5 mini blots
Mouse Total IgG ELISA Kit F049 Bioprocess contaminant assay, 96 tests
Mouse IgG1 ELISA Kit F045 Bioprocess contaminant assay, 96 tests
Mouse IgG2a ELISA Kit F046 Bioprocess contaminant assay, 96 tests
Mouse IgG2b ELISA Kit F047 Bioprocess contaminant assay, 96 tests
Mouse IgM ELISA Kit F090 Bioprocess contaminant assay, 96 tests
MRC5 HCP ELISA Kit F300 Host cell protein assay, 96 tests
NS/0 HCP ELISA kit F220 Host cell protein assay, 96 tests
NS/0 HCP Western Blot kit F225 Host cell protein assay, 5 mini blots
PER.C6 cell line HCP ELISA Kit F530 Host cell protein assay, 96 tests
Pichia pastoris HCP ELISA Kit F140 Host cell protein assay, 96 tests
P.pastoris HCP Western Blot Kit F145 Host cell protein assay, 5 mini blots
Pichia pastoris HCP ELISA Kit, 2nd Generation F640 Antibodies to glycosylation epitopes have been removed and is much more specific than the F140.
Protein A ELISA Kit F050 Bioprocess contaminant assay, 96 tests
Protein A ELISA for human IgG F050H Bioprocess contaminant assay, 96 tests
Protein A ELISA kit F400 Bioprocess contaminant assay, 96 tests
Ps. fluorescens HCP ELISA kit F450 Host cell protein assay, 96 tests
S. cerevisiae HCP ELISA Kit F135 Host cell protein assay, 96 tests
S. cerevisiae HCP Western Blot Kit F130 Western Blot assay, 5 mini blots
SP 2/0 HCP ELISA Kit F180 Host cell protein assay, 96 tests
SP 2/0 HCP Western Blot Kit F185 Western Blot assay, 5 mini blots
S.aureus HCP ELISA Kit F320 Host cell protein assay, 96 tests
Ultra-Sensitive Insulin ELISA Kit F280 Bioprocess contaminant assay, 96 tests
Vero cell HCP ELISA kit F500 Host cell protein assay, 96 tests
Polyclonal Antisera
A549 HCP A549 Goat, neat serum
A549 HCP A549-PA Goat, IgG fraction
A549 HCP A549-AF Goat, affinity purified
Bovine Plasma Proteins BPP04 Rabbit, neat serum
Bovine Plasma Proteins BPP04-PA Rabbit, IgG fraction
Bovine Plasma Proteins BPP04-AF Rabbit, affinity purified
BHK HCP BHK1107 Goat, neat serum
BHK HCP BHK1107-PA Goat, IgG fraction
BHK HCP BHK1107-AF Goat, affinity purified
Campylobacter species 9-25B-PA Rabbit, IgG fraction
Campylobacter species 9-61A Guinea Pig, neat serum
Chlamydia species 8-29A Rabbit, neat serum
Chlamydia species 8-29A-PA Rabbit, IgG fraction
Chlamydia species 8-48 Guinea Pig, neat serum
CHO HCP C0016 Goat, neat serum
CHO HCP C0016-PA Goat, IgG fraction
CHO HCP C0016-AF Goat, affinity purified
CHO-CM HCP M0016 Goat, neat serum
CHO-CM HCP M0016-PA Goat, IgG fraction
CHO-CM HCP M0016-AF Goat, affinity purified
CHO HCP 3G 3G-0016 Goat, neat serum
CHO HCP 3G 3G-0016PA Goat, IgG fraction
CHO HCP 3G 3G-0016AF Goat, affinity purified
Cytomegalovirus 8-21B Rabbit, neat serum
Cytomegalovirus 8-53A Guinea Pig, neat serum
E. coli HCP F117 Goat, neat serum
E. coli HCP F117-PA Goat, IgG fraction
E. coli HCP AP117 Goat, affinity purified
Haemophilus influenzae 8-21A Rabbit, neat serum
Haemophilus influenzae 8-21A-PA Rabbit, IgG fraction
Haemophilus influenzae 8-53B Guinea Pig, neat serum
Haemophilus influenzae 8-53B-PA Guinea Pig, IgG fraction
HEK 293 HCP HK293 Goat, neat serum
Herpes Simplex Virus 8-25A Rabbit, neat serum
Herpes Simplex Virus 8-25A-PA Rabbit, IgG fraction
Herpes Simplex Virus 8-40B Guinea Pig, neat serum
Klebsiella pneumoniae, Genus 9-01 Rabbit, neat serum
Lactococcus lactic HCP LL5557 Goat, neat serum
Lactococcus lactic HCP LL5557-PA Goat, IgG fraction
Lactococcus lactic HCP LL5557-AF Goat, affinity purified
MRC5 HCP MRC5 Goat, neat serum
MRC5 HCP MRC5-PA Goat, IgG fraction
MRC5 HCP MRC5-AF Goat, affinity purified
Mycobacterium genus 8-27B Rabbit, neat serum
Mycobacterium genus 1-04-PA Guinea Pig, IgG fraction
Neisseria meningitidis 8-24B Rabbit, neat serum
Neisseria meningitidis 8-36 Guinea Pig, neat serum
NS/0 HCP N9192 Goat, neat serum
NS/0 HCP N9192-PA Goat, IgG fraction
NS/0 HCP N9192-AF Goat, affinity purified
PER.C6 cell line HCP PC118 Goat, neat serum
PER.C6 cell line HCP PC118-PG Goat, IgG fraction
PER.C6 cell line HCP PC118-AF Goat, affinity purified
Pichia pastoris HCP PI-49 Goat, neat serum
Pichia pastoris HCP PI-49PA Goat, IgG fraction
Pichia pastoris HCP PI-49AF Goat, affinity purified
Pichia pastoris HCP PI-49AF-B Goat, affinity purified-biotinylated
Pichia Pastoris HCP PI-49AF-AP Goat, affinity purified-alk. phosphatase
Pseudomonas fluorescens 7-04 Goat, neat serum
Pseudomonas fluorescens 7-04PA Goat, IgG fraction
Pseudomonas fluorescens 7-04AF Goat, affinity purified
Pseudomonas species 8-57 Guinea Pig, neat serum
Saccharomyces cerevisiae HCP 82-SC Goat, neat serum
Saccharomyces cerevisiae HCP 82SC-PA Goat, IgG fraction
Saccharomyces cerevisiae HCP 82SC-AF Goat, affinity purified
Shigella species 9-03-PA Rabbit, IgG fraction
Shigella species 9-04 Rabbit, neat serum
SF9 HCP 12-SF9 Rabbit, neat serum
SF9 HCP 12-SF9-PA Rabbit, IgG fraction
SF21 HCP 34-SF21 Rabbit, neat serum
SF21 HCP 34-SF21-PA Rabbit, IgG fraction
SP2/0 HCP SP110 Goat, neat serum
SP2/0 HCP SP110-PA Goat, IgG fraction
SP2/0 HCP SP110-AF Goat, affinity purified
SP2/0 HCP SP110AF-B Goat, affinity purified-biotinylated
SP2/0 HCP SP110AF-AP Goat, affinity purified-alk. phosphatase
S. aureus HCP SA8114 Goat, neat serum
S. aureus HCP SA8114-AF Goat, affinity purified
Streptococcus pneumoniae 8-31B Rabbit, neat serum
Streptococcus pneumoniae 8-42A Guinea Pig, neat serum
Streptococcus pneumoniae 8-43A-PA Guinea Pig, IgG fraction
Total Goat Milk Proteins 4042 Rabbit, neat serum
Total Goat Milk Proteins 4042-PA Rabbit, IgG fraction
Total Goat Milk Proteins 4042-AF Rabbit, affinity purified
Vero cell HCP VC807 Goat, neat serum
Vero cell HCP VC807-PA Goat, IgG fraction
Vero cell HCP VC807-AF Goat, affinity purified
Stab-ELISA-rÔ Enzyme Conjugate Diluent/Stabilizers
Alkaline Phosphatase I-045 500mL
Alkaline Phosphatase I-040 1000mL
Alk Phos w/ HAMA blocker I-045H 500mL
Alk Phos w/ HAMA blocker I-040H 1000mL
Horseradish Peroxidase I-035 500mL
Horseradish Peroxidase I-030 1000mL
HRP w/ HAMA blocker I-035H 500mL
HRP w/ HAMA blocker I-030H 1000mL
Liquid Substrates
PNPP Liquid Substrate F008 For use with Alk Phos in ELISA, 100mL
PNPP Liquid Substrate F10008 For use with Alk Phos in ELISA, 1L
TMB Liquid Substrate F027 For use with HRP in ELISA, 100mL
TMB Liquid Substrate F100027 For use with HRP in ELISA, 1L
TMB Liquid Substrate F129 For use with HRP in Western Blot, 100mL
TMB Liquid Substrate F100029 For use with HRP in Western Blot, 1L
BCIP/NBT Liquid Substrate F064 For use with Alk Phos in WB, 1 reagent, 100mL
BCIP/NBT Liquid Substrate F00064 For use with Alk Phos in WB, 1 reagent, 1L
Sample Dilution Buffers
Sample Dilution Buffer, 1L, 100mL, 500mL I028 I028-100 I028-500 For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits
A549 Sample Diluent, 100mL , 500mL F233A-100 F233A-500 For use with A549 HCP ELISA kit
BSA Sample Dilution Buffer, 1L , 100mL , 500mL F031A / F031A-100 / F031A-500 For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits
Goat IgG Dilution Buffer, 1L , 100mL , 500mL F213A /F213A-100 / F213A-500 For use with Goat IgG ELISA kit
Goat Milk Diluent Buffer, 1L , 100mL r, 500mL F243A /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit
NS/0 Sample Dilution Buffer, 1L ,100mL ,500mL F223A / F223A-100 /F223A-500 For use with NS/0 HCP ELISA kit
SP2/0 Sample Dilution Buffer, 1L , 100mL , 500mL F183A / F183A-100 / F183A-500 For use with SP2/0 HCP ELISA kit
Immunogenicity Products
Passive Coated Immunogenicity Kit IM10 Kit for immunogenicity testing
Biotinylated Antigen Coated Immunogenicity Kit IM20 Kit for immunogenicity testing
Biotinylation Kit IM30
Human Immunoglobulin Screening Kit IM40 Kit for immunogenicity testing
Human IgG Subclass Kit IM50 Kit for immunogenicity testing
Substrate Kit IM109 TMB & Stop Solution for ELISA, 50mL
Coatingate Buffer, 1L IM101 For immunogenicity testing
Conjugate Diluent, 1L IM103 For immunogenicity testing
Conjugate Diluent, 500mL IM103-500 For immunogenicity testing
Sample Diluent, 500mL IM104 For immunogenicity testing
FG Blocker, 1L IM107 For immunogenicity testing
BSA Antigen, 1mg/mL IM112 For immunogenicity testing
Bovine IgG Antigen, 1mg/mL IM113 For immunogenicity testing
Bovine Transferrin Antigen, 1mg/mL IM114 For immunogenicity testing
CHO HCP Antigen, 1mg/mL IM115 For immunogenicity testing
E. coli HCP Antigen, 1mg/mL IM116 For immunogenicity testing
Goat IgG Antigen, 1mg/mL IM117 For immunogenicity testing
HSA Antigen, 1mg/mL IM118 For immunogenicity testing
Human IgD Antigen, 1mg/mL IM141 For immunogenicity testing
Human Transferrin Antigen, 1mg/mL IM119 For immunogenicity testing
Insulin Antigen, 1mg/mL IM121 For immunogenicity testing
Mouse IgG Antigen, 1mg/mL IM122 For immunogenicity testing
NS/0 HCP Antigen, 1mg/mL IM123 For immunogenicity testing
SP2/0 HCP Antigen, 1mg/mL IM125 For immunogenicity testing
Gyros Kits-discontinued
CHO 3G HCP G550 For use on the Gyros xP workstation, 560 tests
CHO 3G HCP G550-1 For use on the Gyros xP workstation, 112 tests
E.coli HCP G410 For use on the Gyros xP workstation, 560 tests
E.coli HCP G410-1 For use on the Gyros xP workstation, 112 tests
Protein A G400 For use on the Gyros xP workstation, 560 tests
Protein A G400-1 For use on the Gyros xP workstation, 112 tests
DNA Kits
DNA Extracton in Wells D100W 96 tests in a deep well microtiter plate
DNA Extraction in Tubes D100T 50 tests in microfuge tubes
Residual CHO Host Cell kit in Wells D550W 96 tests in a deep well microtiter plate
Residual CHO Host Cell kit in Tubes D550T 50 tests in microfuge tubes
CHO Amplification kit in Wells D555W 96 tests in a deep well microtiter plate
CHO Amplification kit in Tubes D555T 50 tests in microfuge tubes
Residual Ecoli Host Cell kit in Wells D410W 96 tests in a deep well microtiter plate
Residual Ecoli Host Cell kit in Tubes D410T 50 tests in microfuge tubes
E.coli Amplification kit in Wells D415W 96 tests in a deep well microtiter plate
E.coli Amplification kit in Tubes D415T 50 tests in microfuge tubes
Residual NS/0 Host Cell kit in Wells D220W 96 tests in a deep well microtiter plate
Residual NS/0 Host Cell kit in Tubes D220T 50 tests in microfuge tubes
Miscellaneous Reagents & Components
Deep Well Extraction Plate w/Sealing Mat D102 5 plates
DNA Extraction Buffer for use in tubes D108 30mL
DNA Extraction Buffer for use in wells D105 30mL
DNA Sample Diluent D006-100 100mL
DNA Sample Diluent D006-500 500mL
DNA Sample Diluent D006-1000 1L
DNA TE Buffer, 1L D001 1L
CHO DNA Concentrate D552 10ug/mL, 1mL
E.coli DNA Concentrate D412 10ug/mL, 1mL
NS/0 DNA Concentrate D222 10ug/mL, 1mL
更多详情请来电: :


产品编号:
市场价:¥0.00元
会员价:¥0.00元
品牌:上海比朗BILON
生产厂家:BILON上海比朗
BILON上海比朗LDZM-60KCS智能型不锈钢立式灭菌器
智能型不锈钢立式灭菌器
●塑料防烫罩壳机身不锈钢材料
●转盘式快开盖结构
●智能化自动控制灭菌循环程序
●设定温度时间采用一键制操作方式
●数码液晶窗中文显示运行状态
●灭菌温度任意设定范围(
●灭菌时间任意设定范围(0-99小时)
●内排汽装置(灭菌结束无蒸汽外排现象)
●安全联锁互动控制
●灭菌终了蜂鸣提醒后自动停机
●具有断水保护控制
●可配打印功能(另外购买)
|
型 号 |
容积 |
电源 |
功率 |
灭菌室尺寸 |
毛重 |
体积(mm) |
备注 |
价格(元) |
|
LDZM-60KCS |
|
220V |
3.5KW |
Φ355×600 |
|
660×780×1150 |
标准配置 |
13860 |
|
蒸汽内排 |
17160 |
|||||||
|
真空干燥 |
19800 |
Propylthiouracil 丙基硫氧嘧啶
货号:BS228-25g
规格:25g
品牌:Jinpan
丙基硫氧嘧啶
|
产品编号 |
产品名称 |
规格 |
|
BS228–25g |
丙基硫氧嘧啶 |
25g |
产品简介:
科研用生化试剂。丙基硫氧嘧啶又称为6-正丙基-2-硫代尿嘧啶、6-PTU,是一种巯基尿嘧啶衍生物,已被证明是一种抗甲状腺机能亢进的药物。已知6-丙基-2-硫氧嘧啶可通过碘原酶I型(DIO1)抑制甲状腺素对三碘原氨酸的去碘化。它是一种甲状腺稳态(甲状腺激素)剂,可作为甲状腺稳态药物分离和检测技术(如毛细管电泳、高效液相色谱或气相色谱)的参考物质。
别名:6-正丙基-2-硫代尿嘧啶;6-丙基-2-巯基-4-羟基嘧啶;6-PTU;6-n-Propyl-2-thiouracil
英文名称:Propylthiouracil
CAS:51-52-5
分子式:C7H10N2OS
分子量:170.23
外观(性状):白色或黄白色粉末
储存条件:RT
纯度:98%
溶解性:10 mg/ml(沸水),16 mg/ml(乙醇),50 mg/ml(1M NaOH)
单位:瓶
注意事项:
1. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
有效期:
室温保存4年。
| 货号 | BS228-25g |
| 规格 | 25g |
| 品牌 | Jinpan |
| 说明书下载 | 点击下载 |
山梨醇 D-Sorbitol
货号: BS115-1kg
规格:1kg
品牌:biosharp
| 货号 | BS115-1kg |
| 规格 | 1kg |
| 品牌 | biosharp |
| 说明书下载 | 点击下载 |

| XC47/11-10型号液氮罐
MVE的解决方案能够最好地优化冻存条件和技术,也为众多用户带来了成功。其产品种类齐全, 性能优异,是各种科研、工业和临床机构液氮冻存和低温运输的不可多得的产品。 产品特征: 1.MVE XC 系列液氮罐的容量自700 – 5000 管及150 至1000 多个冻存管。 2.产品持久耐用、重量轻,制造技术世界领先,并有5 年的真空保证,诸多优势 使MVE系列产品成为大多数需求中可依赖的产品。 3、XC 系列产品专为那些需要大容量储存及低液氮消耗的用户设计 |
–
|
A耐用且防干扰的盖子设计 B 易维护的盖子设计 C 高强度,低重量的铝质结构 D 高强度的颈形管减少了液氮损失 E 锁定带 |
配件 •脚轮基座 •备用软木塞 •罐 •液位探测棒 •传输软管 •方形架 ( 限47/11-6) •液位报警 •分相器 •塑料盒 |
| 商品属性 | |
|---|---|
| 商品名称 | MVE XC47/11-10型号液氮罐-XC47/11-10-MVE |
| 型号 | XC47/11-10 |
| 类别 | 生命科学仪器|||低温存储|||储存型液氮罐|||MVEXC47/11-10型号液氮罐 |
| 品牌 | MVE |
| 品牌简介 | MVE |
| 关键字 | 液氮罐 液氮瓶 生物存储罐 生物容器 杜瓦瓶 自增压液氮罐 低压,液氮,提桶,产品,盖子,容积,耐用 |
透明质酸酶Hyaluronidase -20度
货号:BS171-100mg
规格:100mg
品牌:Jinpan
| 货号 | BS171-100mg |
| 规格 | 100mg |
| 品牌 | Jinpan |
| 说明书下载 | 点击下载 |
Oritavancin (diphosphate);奥利万星二磷酸盐
| 别名 | LY333328diphosphate |
| CAS | 192564-14-0 |
| 分子式 | C86H103Cl3N10O34P2 |
| 分子量 | 1989.09 |
| 纯度 | HPLC≥98% |
| SMILES | O[C@@H]1[C@@H](O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@]2(C)NCC3=CC=C(C4=CC=C(Cl)C=C4)C=C3)[C@@H](O[C@H](CO)[C@H]1O)OC5=C(OC6=CC=C([C@H]([C@H]7NC([C@H](NC)CC(C)C)=O)O)C=C6Cl)C=C([C@](NC([C@@H](NC7=O)CC(N)=O)=O)([H])C(N[C@](C8=O)([H])C9=CC%10=C(O)C=C9)=O)C=C5OC%11=CC=C([C@H]([C@](C(N[C@@](C(O)=O)([H])C%12=C%10C(O)=CC(O)=C%12)=O)([H])N8)O[C@@](O[C@@H](C)[C@@H]%13O)([H])C[C@@]%13(N)C)C=C%11Cl.O=P(O)(O)O.O=P(O)(O)O |
| 靶点 | Bacterial |
| 规格 | 1mg 5mg 10mg |
是一种新型糖肽抗生素, 可用于治疗革兰氏阳性菌感染。
【简单介绍】
【详细说明】

CHIR-99021 Hydrochloride
| 有效期 | 2年 |
| MDL | MFCD30489757 |
| 别名 | CT-99021 |
| CAS | 1797989-42-4 |
| 分子式 | C22H18Cl2N8·HCl |
| 分子量 | 501.80 |
| 储存条件 | -20℃ |
| 纯度 | ≥98% |
| 外观(性状) | Powder |
| 单位 | 瓶 |
| 生物活性 | CHIR-99021的盐酸盐, 是GSK-3α/β抑制剂,IC50为10 nM/6.7 nM。其对GSK-3的选择性比对Cdc2和ERK2高500倍以上。CHIR-99021 是一种有效的药理性的 Wnt/beta-catenin 信号通路的激活剂。CHIR-99021 可显著地拯救光诱导的 autophagy,并可增强 GR,RORα和自噬相关蛋白等表达。[1-3] |
| In Vitro | CHIR-99021作用于GSK-3比作用于其最接近的同系物CDC2和ERK2,及其他蛋白激酶选择性高500倍。此外,CHIR-99021只与一组22种药理相关的受体微弱结合,且对一组23种非激酶的酶几乎没有抑制活性。CHIR-99021作用于表达胰岛素受体的CHO-IR细胞,诱导糖原合成酶(GS)激活,EC50为0.763 μM。[1]CHIR-99021(3 μM)作用于3T3-L1前脂肪细胞,除了模拟胰岛素的作用,抑制GSK-3,使胞内游离β-catenin增加1.9倍,模仿经典Wnt信号通路。在分化的前3天,CHIR-99021处理,抑制脂肪细胞分化,IC50为0.3 μM,通过抑制CCAAT/增强子结合蛋白α(C/EBPα)和过氧化物酶体增殖物激活受体γ(PPARγ)的诱导作用。[2] 不像LiCl和AR-A014418,CHIR-99021处理,即使在高浓度,不会降低INS-1E细胞活力。相反,CHIR-99021增加INS-1E细胞增殖,这种作用具有剂量依赖性,且显著抑制高糖和高棕榈酸诱导的INS-E细胞死亡,这种作用具有浓度依赖性。CHIR-99021浓度低至1 μM,处理离体大鼠胰岛,促进β细胞复制,5 μM CHIR-99021处理,使细胞复制增长2-3倍。[3] |
| In Vivo | CHIR-99021按30 mg/kg 剂量口服处理患2型糖尿病的啮齿类动物模型,增强葡萄糖代谢,处理3-4小时后,最大血浆葡萄糖减少近150 mg/dl,而血浆胰岛素保持或低于对照水平。CHIR-99021按16 或 48 mg/kg剂量口服给药ZDF大鼠,1小时后,再口服葡萄糖,显著改善糖耐量,16 mg/kg 和 48 mg/kg处理组的血浆葡萄糖分别减少14%和33%,较高剂量CHIR-99021处理,再口服葡萄糖,还可以降低高血糖。[1]CHIR-99021处理移植小鼠或人造血干细胞(HSC)的受体小鼠,显著增强造血重建功能,说明GSK-3是一种特异性的HSC活性调节剂。[4] |
| SMILES | N#CC1=CC=C(NCCNC2=NC=C(C3=NC=C(C)N3)C(C4=CC=C(Cl)C=C4Cl)=N2)N=C1.[H]Cl |
| 靶点 | GSK-3α,GSK-3β |
| 动物实验 | Animal Models: 雌性db/db小鼠或雄性2型糖尿病ZDF大鼠; Dosages: ~48 mg/kg; Administration: 口服处理[1] |
| 数据来源文献 | [1] Ring DB, et al. Diabetes, 2003, 52(3), 588-595. [2] Bennett CN, et al. J Biol Chem, 2002, 277(34), 30998-31004. [3] Mussmann R, et al. J Biol Chem, 2007, 282(16), 12030-12037 |
| 规格 | 5mg |
是一个GSK-3α/β的抑制剂。