FIGURE 1：

CUT&RUN gene browser tracks. CUT&RUN was performed as described above. Data verifies low non-specific MNase digestion with the absence of peaks in the IgG track, an expected H3K4me3 profile with sharp promoter peaks, and broad peaks in heterochromatin regions consistent with H3K27me3. Image was generated using the Integrative Genomics Viewer (IGV, Broad Institute).

FIGURE 2：

Size distribution of released chromatin. CUT&RUN was performed as described above. Excised DNA is highly enriched for mononucleosomes (peaks at ~300 bp represent 150 bp nucleosomes + sequencing adapters).

FIGURE 3：

CUT&RUN genome-wide heatmaps. CUT&RUN was performed as described above. Heatmaps show CUT&RUN signal aligned to annotated transcription start sites (TSS, +/- 2kb). High and low signal are ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. Gene rows in each heatmap are aligned and sorted from high to low signal relative to H3K4me3 (middle).

FIGURE 4：

Protein gel data. CUTANATM pAG-MNase (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

货号

产品名称

规格

15-1016

CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

50 Reactions

15-1116

CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

250 Reactions

FIGURE 1: 

Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained  with Coomassie blue. The migration and molecular  weight of the protein standards are indicated. Uncharged  pAG-Tn5 monomer is 78.5 kDa, however once charged  with DNA, Tn5 dimerizes to a final complex weight of 191 kDa.

FIGURE 2:

Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

FIGURE 3: 

CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control.

货号

产品名称

规格

15-1017

CUTANA™ pAG-Tn5 for CUT&Tag

50 Reactions

15-1117

CUTANA™ pAG-Tn5 for CUT&Tag

250 Reactions