StemRegenin 1 increases the number of CD34+ cells after 5 to 7 days with an EC50 of 120 nM. Culture of mPB CD34+ cells with cytokines plus SR1 (1 μM) for 7 days increases the number of CD34+, CD133+, and CD90+ hematopoietic stem and progenitor cell populations 2.6-, 2.3-, and 10-fold, respectively compared to control cells. Continued culture with SR1 (1 μM) for 3 weeks leads to an 11-fold increase in total nucleated cells (TNC), a 73-fold increase in CD34+ cells as compared to control cultures, and a 1118-fold increase in CD34+ cells relative to input cells. Culture of 1×103 cord blood CD34+ cells for 5 weeks with SR1 (1 μM) results in the production of 1.69×106 colony forming cells. SR1-induces CD34+ cell expansion acts by binding and antagonizing AhR as evident by decreased CYP1B1 and AHRR mRNA levels. SR1 (1 μM) treatment accelerates the proliferation of CD34+ cells and decreased the expression levels of VentX in human CD34+ cells. Ectopic expression of VentX prevents SR1-induced expansion of CD34+ cells. Sequential coculture with bone morphogenetic protein 4 (20 ng/mL), PGE2 (2 μM), and SR1 (0.75 μM) lead to robust Macaca nemestrina iPSC hematopoietic progenitor cell formation. CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of CD34 expression and cultured in SR1 (0.75 μM) expands 3-fold and maintained this long-term repopulating HSC phenotype.
StemRegenin-1（SR1）常见问题：
1. 如何使用SR1，使用浓度多少？
大多数培养条件下，稀释SR1储存液（如10 mM DMSO） 1:10,000，加入感兴趣的造血干细胞培养基内获得工作液浓度为1 μM。微量浓度的DMSO（0.01%）对细胞培养物无副作用。但对于非传统的培养基，如含高浓度人血清的培养基，建议进行一组不同浓度SR1预实验，因为SR1的蛋白结合特性仍未知。
M307 PROTEASE INHIBITOR COCKTAIL, PLANT Grade:PROTEOMICS GRADE CAS Number: N/ASynonym(s): None Molecular Weight: N/AMolecular Formula: N/A Storage Condition: FROZEN CodeSizePriceQuantity M307-1ML Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.
