货号：22582，名称： Ellman’s Reagent, 5,5′-Dithio-bis-（2-nitrobenzoic Acid） ，规格：5 GM
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Ellman’s Reagent, 5,5′-Dithio-bis-（2-nitrobenzoic Acid）介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

Ellman’s Reagent, 5,5′-Dithio-bis-（2-nitrobenzoic Acid）现货供应

Ellman’s Reagent, 5,5′-Dithio-bis-（2-nitrobenzoic Acid）品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

主要产品包括：

1）化学发光体和比色底物

2）分析试剂

3）荧光标记探针

Thermo Scientific Pierce DTNB （Ellman’s Reagent） reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Ellman’s Reagent （5,5′-dithio-bis-[2-nitrobenzoic acid]） is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB （14,150M-1cm-1 at 412nm）. Ellman’s Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.

Highlights:

• Determine availability of sulfhydryls for labeling or crosslinking applications
• Assay by measuring the absorbance at 412nm or using cysteine as a standard
• Temporarily block sulfhydryls to perform chemistries on other protein functionalities

 Ellman’s Reagent, 5,5′-Dithio-bis-（2-nitrobenzoic Acid）仅用于科研

4）酶联免疫分析试剂盒等