产品名称

RecombiMAb anti-mouse PD-L1 (B7-H1) (D265A)

产品货号

CP001

产品规格

1mg

反应种属

Mouse

克隆号

10F.9G2™-CP001

同种型

Mouse IgG1(switched from rat IgG2b)

免疫原

Mouse CD274

实验应用

in vivo PD-L1 blockade*

Immunofluorescence*

Immunohistochemistry (frozen)*

Flow cytometry*

Western blot*

*Reported for the original rat IgG2b 10F.9G2 antibody

产品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

无菌处理

0.2 µm filtration

纯化方式

Protein A

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐抗体稀释液

InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

应用

文章

体内PD-L1阻断

(in vivo PD-L1 blockade)

1. Grasselly, C., et al. (2018). ‘The Antitumor Activity of Combinations of Cytotoxic 

Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent’ Front 

Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). ‘PD-1 Inhibitory Receptor Downregulates Asparaginyl 

Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory 

T Cells’ Immunity 49(2): 247-263 e247.

3. Jaworska, K., et al. (2015). ‘Both PD-1 ligands protect the kidney from ischemia 

reperfusion injury’ J Immunol 194(1): 325-333.

4. Kim, J., et al. (2015). ‘Memory programming in CD8(+) T-cell differentiation is 

intrinsic and is not determined by CD4 help’ Nat Commun 6: 7994.

5. Zander, R. A., et al. (2015). ‘PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk 

Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity’ Cell 

Host Microbe 17(5): 628-641.

体内PD-L1阻断，流式细胞术

(in vivo PD-L1 blockade, Flow 

Cytometry)

1. Aloulou, M., et al. (2016). ‘Follicular regulatory T cells can be specific for the 

immunizing antigen and derive from naive T cells’ Nat Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). ‘A Threshold Level of Intratumor CD8+ T-cell PD1 

Expression Dictates Therapeutic Response to Anti-PD1′ Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). ‘Highly pathological influenza A virus infection is 

associated with augmented expression of PD-1 by functionally compromised 

virus-specific CD8+ T cells’ J Virol 88(3): 1636-1651.

体内PD-L1阻断，免疫荧光

(in vivo PD-L1 blockade, 

Immunofluorescence)

1.Willimsky, G., et al. (2013). ‘Virus-induced hepatocellular carcinomas cause 

antigen-specific local tolerance’ J Clin Invest 123(3): 1032-1043.

免疫组织化学（冷冻），免疫荧光

(Immunohistochemistry (frozen), 

Immunofluorescence)

1.Riella, L. V., et al. (2011). ‘Essential role of PDL1 expression on nonhematopoietic 

donor cells in acquired tolerance to vascularized cardiac allografts’ Am J Transplant 

11(4): 832-840.