将3.5×105 Gen2.2细胞或Flt3-DC在96孔板中孵育1小时,不含或不含指定浓度的抑制剂,然后用1μMCpG(A型或B型)或1μg/ mL CL097刺激或R848。 5或12小时后,收集细胞培养物上清液,通过离心澄清,并在-80℃冷冻直至分析细胞因子水平。对于细胞活力测定,未受刺激的细胞在不存在或存在抑制剂的情况下孵育12小时。然后固定细胞并通过流式细胞术分析活细胞的百分比。
数据来源文献
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