用途： 

   通过 8 周 3 针的标准免疫程序制备兔子多克隆抗体， ELISA 滴度 （Cutoff 值为 0.1000）可达到 1:10000-1:1000000。

不会引出像FCA那样强的免疫应答，需要强免疫原。

Description：

Imject Alum, 50mL, contains an aqueous solution of aluminum hydroxide (40mg/mL) and magnesium hydroxide (40mg/mL) plus inactive stabilizers
Storage: Upon receipt store at room temperature. Product shipped at ambient temperature.
Note: This product is supplied for research or manufacturing applications only and is not intended for clinical use.
Introduction：
Generating a strong immune response with immunogens can be a slow and inefficient process. Adding an adjuvant, such as Thermo Scientific Imject Alum, to the antigen stimulates an improved immune response compared to the antigen alone. Adjuvants increase the immune response by localizing antigen for an extended time and attracting the appropriate cells (T cells, B cells and APC) to interact with the immunogen and each other. Adjuvants are mixed and injected along with antigen to prevent catabolism.
Alum is frequently used as an alternative to Freund’s adjuvants because alum is less hazardous. Although Freund’s complete and incomplete adjuvants produce a stronger, longer lasting immunogenic response compared to other adjuvants, they are hazardous to the researcher and can produce lesions at the injection site.
A traditional drawback when using alum is the reagent preparation time; however, Imject Alum is preformulated and simply requires mixing with the antigen and then injection into the animal. Imject Alum provides an effective and convenient alternative to the more hazardous Freund’s reagents. This product can be used with any immunogen kit for preparing hapten-carrier conjugates.
Protocol for Mixing Immunogen with the Imject Alum
Note: To avoid anaphylaxis, do not use adjuvants for intravenous injection.
1.Shake the capped bottle of Imject Alum well before use.
2.Add Imject Alum dropwise with constant mixing to the immunogen solution so the final volume ratio of Imject Alum to immunogen is 1:1 (e.g., add 100μL of Imject Alum to 100μL of immunogen) to 1:3 (e.g., add 100μL of Imject Alum to 300μL of immunogen solution).
3.Continue mixing for 30 minutes after adding the Imject Alum. Mixing allows the Imject Alum to effectively adsorb antigen.
4.Immunize animal according to standard protocol (see Harlow and Lane, 1988, for more information). The immunogen concentration before mixing with alum and the species being tested will determine injection amount. Generally, 50-100μg of immunogen is used per mouse per immunization. Rabbits require 100μg of immunogen per injection at each subcutaneous site. For rabbit or mouse, a typical concentration of immunogen (before mixing with Imject Alum) is 100μg per 100μL.