(-)-Epigallocatechin (-)-表棓儿茶素 标准品
| CAS | 970-74-1 |
| 分子式 | C15H14O7 |
| 分子量 | 306.279999 |
| 储存条件 | Store in dry and dark place. |
| 纯度 | HPLC : ≥ 98% |
| 单位 | 瓶 |
| 货期 | 4周 |
| 规格 | 20mg |
(-)-Epigallocatechin gallate (-)-表儿茶素酸酯 标准品
| CAS | 989-51-5 |
| 分子式 | C22H18O11 |
| 分子量 | 458.369995 |
| 储存条件 | Store in dry and dark place. |
| 纯度 | HPLC : ≥ 98% |
| 单位 | 瓶 |
| 货期 | 4周 |
| 规格 | 20mg |
(-)-Epigallocatechin 表没食子儿茶素
| MDL | MFCD00075939 |
| EC | EINECS 619-254-4 |
| 别名 | 绿茶儿茶酚; EGC |
| CAS | 970-74-1 |
| 分子式 | C15H14O7 |
| 分子量 | 306.27 |
| 纯度 | HPLC≥98% |
| 单位 | 瓶 |
| 生物活性 | (-)-Epigallocatechin (Epigallocatechin) is the most abundant flavonoid in green tea, can bind to unfolded native polypeptides and prevent conversion to amyloid fibrils.(-)-Epigallocatechin, widespread in plants, has been shown to exhibit anti-tumor, anti-cancer and anti-inflammatory functions.[1-4] |
| In Vitro | (-)-Epigallocatechin (EGC) is a potent inhibitor of amyloidogenic cystatin I66Q amyloid fibril formation in vitro. Computational analysis suggests that (-)-Epigallocatechin prevents amyloidogenic cystatin fibril formation by stabilizing the molecule in its native-like state as opposed to redirecting aggregation to disordered, amorphous aggregates [1]. Combined curcumin and EGCG treatment reduced the cancer stem-like Cluster of differentiation 44 (CD44)-positive cell population. Western blot and immunoprecipitation analyses revealed that curcumin and (-)-Epigallocatechin (EGC) specifically inhibited STAT3 phosphorylation and STAT3-NFkB interaction was retained [2]. (-)-Epigallocatechin (EGC) exhibits a MIC and MBC of 5 μg/mL and 20 μg/mL respectively and effectively eradicated E. faecalis biofilms. (-)-Epigallocatechin induces the formation of hydroxyl radicals in E. faecalis. The addition of DIP protected E. faecalis against EGCG-mediated antibacterial effects. At sub-MIC, (-)-Epigallocatechin induces significant down-regulation of E. faecalis virulence genes [3].(-)-Epigallocatechin (EGC) shows the anti-proliferation effects on AML cells. EGC-treated cells show not only the suppression of FLT3, but also the suppression of phosphorylation of MAPK, AKT and STAT5. EGC suppresses FLT3 expression through Hsp90[4]. |
| SMILES | O[C@H]1[C@@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1 |
| 靶点 | MMP |
| 细胞实验 | Cell lines: MOLM-13, MOLM-14, MV4-11 and KOCL-48 cells；Concentrations: 0-200 μM；Incubation Time: 72 h；Method: Cell proliferation is determined by trypan blue dye exclusion test. Briefly, cells are seeded in 6-well plates at a density of 1×105 cells/ml in the presence of different concentrations of EGCG, EGC, ECG and C for 72 hours. After the treatment, 10 μl of the cell suspension is mixed with 10 μl of 0.4% trypan blue, and alive cells are counted manually using a hemacytometer.[4] |
| 数据来源文献 | [1]. Wang N, et al. (-)-Epigallocatechin-3-gallate Inhibits Fibrillogenesis of Chicken Cystatin. J Agric Food Chem. 2015 Jan 26 [2]. Chung SS, et al. Curcumin and Epigallocatechin Gallate Inhibit the Cancer Stem Cell Phenotype via Down-regulation of STAT3-NFκB Signaling. Anticancer Res. 2015 Jan;35(1):39-46. [3]. Lee P, et al. Effects of Epigallocatechin gallate against Enterococcus faecalis biofilm and virulence. Arch Oral Biol. 2015 Mar;60(3):393-9. [4] Ly BT, et al. PLoS One. 2013, 8(6):e66378. |
| 规格 | 5mg 10mg |
是一种类黄酮，能结合未折叠多肽和防止转化为淀粉样纤维。
(-)-Epigallocatechin Gallate 表没食子儿茶素没食子酸酯
| EC | EINECS 479-560-7 |
| MDL | MFCD00075940 |
| 别名 | TEACATECHIN;EGCG |
| 英文名称 | (-)-Epigallocatechin Gallate |
| CAS | 989-51-5 |
| 分子式 | C22H18O11 |
| 分子量 | 458.38 |
| 纯度 | HPLC≥98% |
| 单位 | 瓶 |
| 生物活性 | (-)-Epigallocatechin Gallate 是一种天然的多酚黄酮类化合物，能够抑制 EGFR，HER2 和 HER3 的活化，具有抗肿瘤活性。[1-3] |
| In Vitro | ( – ) – 表没食子儿茶素没食子酸酯 (EGCG) 抑制SW837人结肠癌细胞系中的活化EGFR, HER2和HER3。 ( – ) – 表没食子儿茶素没食子酸酯 (10μM) 也抑制细胞生长, 抑制EGFR, HER2和HER3的活化, 并导致COX-2和Bcl-xL蛋白水平降低, 以及治疗96 h后细胞凋亡[1] ]。 ( – ) – 表没食子儿茶素没食子酸酯 (0-35μg/ mL) 抑制结肠直肠癌细胞的增殖。 ( – ) – 表没食子儿茶素没食子酸酯 (35μg/ mL) 诱导结直肠癌细胞凋亡[2]。 ( – ) – 表没食子儿茶素没食子酸酯 (EGCG; 50, 75和100μM) 剂量依赖性地抑制HepG2细胞的生长, 并诱导HepG2细胞凋亡[3]。 |
| In Vivo | ( – ) – 表没食子儿茶素没食子酸酯 (5, 10和20 mg / kg, po) 抑制小鼠原位结直肠癌细胞的生长[2]。 |
| SMILES | O=C(O[C@H]1[C@@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4 |
| 靶点 | Autophagy |
| 动物实验 | 小鼠[2]术后2周, 40只裸鼠中有39只出现肿瘤。根据肿瘤体积, 将39只肿瘤小鼠分为4组：对照组 (n = 9) ; 接受5mg / kg ( – ) – 表没食子儿茶素没食子酸酯 (n = 10) 的组; 接受10mg / kg ( – ) – 表没食子儿茶素没食子酸酯 (n = 10) 的组; 和接受20mg / kg ( – ) – 表没食子儿茶素没食子酸酯 (n = 10) 的组。在治疗组中, ( – ) – 表没食子儿茶素没食子酸酯通过胃内给药, 并且在对照组中, 100μL生理盐水通过胃内给药, 每天一次, 持续14天。用 ( – ) – 表没食子儿茶素没食子酸酯处理小鼠4周后, 使用荧光成像系统连续监测原发性肿瘤的生长和转移。 4周后, 称重原发肿瘤并立即放入液氮 (-196℃) 中, 2至3小时后, 将这些样品保存在-80℃。此外, 原发肿瘤和转移瘤的其他部位固定在4％甲醛中[2]。 |
| 细胞实验 | 将LoVo, SW480, HCT-8和HT-29细胞以5×103细胞的浓度接种于96孔板中; 每个细胞系完全接种在12个孔中。向孔中加入完全培养基, 最高200μL; 培养基含有0μg/ mL, 10μg/ mL, 20μg/ mL和35μg/ mL ( – ) – 表没食子儿茶素没食子酸酯。抑制率= [1 – ( ( – ) – 表没食子儿茶素没食子酸酯基团的吸光度 – 对照组的吸光度) / (对照组的吸光度 – 空白对照组的吸光度) ]×100 [2]。 |
| 数据来源文献 | [1]. De Amicis F, et al. Epigallocatechin gallate inhibits growth and Epithelial-to-Mesenchymal Transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Apr 1.
[2]. Jin H, et al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013; 6:145-53. [3]. Wing Pui Tsang, et al. Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells. The Journal of Nutritional Biochemistry. 2010, 21 (2) : 140-146 |
| 备注 | 以上数据均来自公开文献, Jinpan暂未进行独立验证, 仅供参考。These protocols are for reference only. Jinpan does not independently validate these methods. |
| 规格 | 20mg 10mM*1mL in DMSO 100mg |
是一种多酚黄酮类化合物，能够抑制 EGFR，HER2 和 HER3 的活化，具有抗肿瘤活性。