A-582941 dihydrochloride is a potent, selective and brain-penetrant partial agonist of α7 nAChR, with Kis of 10.8 and 16.7 nM in rat brain membranes and human frontal cortex, respectively. A-582941 dihydrochloride also binds to human 5-HT3 receptor with a Ki of 150 nM. A-582941 has the potential for cognitive deficits associated with various neurodegenerative and psychiatric disorders research.
纯度
>97%
储存/保存方法
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
Pyrithioxin dihydrochloride is a neurodynamic compound, combined with a short period of hyperventilation (HV) was applied in cerebral infarct patients with Hemiplegia.[1]
In Vivo
Chronic treatment (15 days) of aged (22 months) female NMRI mice with pyritinol (200 mg/kg) restored the reduced density of N-methyl-D-aspartate receptors in the aged mouse brain. [2]
[1]. Stoica E, et al. Facilitation through hyperventilation of therapeutic effect of pyrithioxin in cerebral infarct patients. Eur Neurol. 1975;13(4):285-303. [2] H Hartmann, et al. Neuropharmacology. 1993 Feb;32(2):119-2
GYKI 52466 hydrochloride is a selective non-competitive allosteric AMPA receptor antagonist. It exhibits anti-proliferative effects in transformed cells. AMPA is a transmembrane glutamate receptor composed of the subunits GluR-1,2,3,4.
应用
A selective non-competitive allosteric AMPA receptor antagonist
纯度
≥98%
储存/保存方法
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
JNJ-37822681 dihydrochloride is a novel, highly selective dopamine D₂ receptor antagonist characterized by a rapid dissociation rate from the dopamine D₂ receptor. JNJ-37822681 dihydrochloride shows a moderate binding affinity for the dopamine D2L receptor with a Ki of 158 nM.
纯度
98%
储存/保存方法
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
基本信息
可溶性/溶解性
DMSO:33.33 mg/mL (74.86 mM)
生物活性
靶点
D2:0.39 mg/kg(ED50), D2:158 nM(Ki)
In vivo(体内研究)
JNJ-37822681 dihydrochloride occupied D2 receptors in rat brain at relatively low doses (ED50 = 0.39 mg/kg) and was effective in animal models of psychosis (e.g., inhibition of apomorphine-induced stereotypy or D-amphetamine/phencyclidine-induced hyperlocomotion)[4].
FFN 206 dihydrochloride is a fluorescent VMAT2 substrate that can be used to detect VMAT2 subcellular sites in cell culture and shows no detectable inhibition of DAT.
纯度
98%
储存/保存方法
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92.
[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82.
[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7
规格
5mg 10mM*1mL in Water 10mg 25mg
Puromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。
使用本产品的应用案例（仅供参考）
In Vitro
Cell（OC cells，2 μg/mL puromycin）
PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Jinpan, Shanghai, China) at a concentration of 800 μg/mL and puromycin (Jinpan, Shanghai, China) at a concentration of 2 μg/mL.
来源文献：Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.
[1]. Yasuhide Hori, et al. Naftopidil, a selective {alpha}1-adrenoceptor antagonist, suppresses human prostate tumor growth by altering interactions between tumor cells and stroma. Cancer Prev Res (Phila) . 2011 Jan; 4 (1) :87-96.
备注
以上数据均来自公开文献, Jinpan暂未进行独立验证, 仅供参考。 These protocols are for reference only. Jinpan does not independently validate these methods.
