标签归档：cd

Anti-CD127 Reference Antibody(PF-06342674)

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Anti-CD127 Reference Antibody(PF-06342674)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素：<1 eu>纯度：>95%
别名
IL-7Ra
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD127 Reference Antibody(PF-06342674) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

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Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
5′-nucleotidase; 5′-NT; 5′-deoxynucleotidase
宿主
Rabbit
反应种属
Human, Mouse, Rat
应用
WB: 1:1000, IHC-P: 1:500-1:1000
免疫原
Synthetic peptide
性能
形式
Liquid
浓度
0.5 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
218-52
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
CD73, also known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosylphosphatidyl inositol (GPI)-anchored cell surface protein that is encoded by NT5E gene. CD73 is widely expressed on different tissues [PubMed: 9553767, PubMed: 18404475] and cell types including, but not limited to the subsets of T cells and B cells [PubMed: 2550543], endothelial cells [PubMed: 9015312, PubMed: 15358667] and epithelial cells [PubMed: 9169488].
细胞定位
Cell membrane
UniProt
P21589

实验结果图

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: A431 whole cell lysate 20 µg Lane 4: HepG2 whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 120s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 68 kDa Exposure time: 180s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 180s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded rat liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Human CD14 ELISA KIT(人的可溶性细胞分化抗原14) ELISA KIT

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Human CD14 ELISA KIT(人的可溶性细胞分化抗原14) ELISA KIT

货号：BQEH-501-96T

规格：96T

品牌：biosharp

 ELISA KIT又称为ELISA试剂盒、酶联免疫吸附测定试剂盒，是一种灵敏度高，特异性强，重复性好的实验方法，且因其试剂稳定、易保存，操作简便、结果判断客观等已广泛应用在免疫学检验的各领域中，得到全世界科研工作者的认可和应用。

ELISA的基本原理是双抗体夹心法，将样本加入预包被了抗原或抗体的酶标板里，然后再加入酶标记的抗原或抗体，从而固相载体上的抗原或抗体与样品中受检物质的量呈一定的比例。加入酶反应的底物后，底物被酶催化成为有色产物，产物的量与样本中受检物质的量直接相关，故可根据呈色的深浅进行定性或定量分析。

Jinpan ELISA KIT覆盖人、小鼠、大鼠、猪、通用等多种物种，产品达1000多种。每一个产品均经过严格的质检，结果稳定，重复性和线性范围好，特异性和灵敏度高，应用广泛，涉及免疫学、神经学、微生物学、细胞生物学、表观遗传学等多个科学领域。

货号 BQEH-501-96T
规格 96T
品牌 biosharp

BioXCell InVivoMAb anti-mouse CD19（货号：BE0150）

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BioXCell InVivoMAb anti-mouse CD19（货号：BE0150）

克隆号1D3单克隆抗体与小鼠CD19(一种免疫球蛋白超家族的B细胞特异性95 kDa跨膜糖蛋白)反应。CD19包含两个细胞外免疫球蛋白样结构域和一个广泛的细胞质尾。它与CD21和CD81一起作为B细胞受体信号的正调节因子。CD19在大多数淋巴瘤和白血病中高度表达，包括一些不表达CD20的早期B细胞恶性肿瘤。由于这些原因，CD19正迅速成为淋巴增生性疾病免疫治疗的一个有吸引力的替代靶点。据报道，克隆号1D3抗体可depleteB细胞，然而，B细胞deplete通常需要用克隆号1D3、抗小鼠B220(克隆RA3.3A1/6.1)、抗小鼠CD22(克隆Cy34.1)和抗大鼠κ轻链(克隆MAR 18.5)抗体的组合来治疗。

产品详情

产品货号

BE0150

产品规格

1/5/25/50/100mg

抗体亚型

Rat IgG2a, κ

推荐同型对照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号：BE0089）

推荐抗体稀释液

InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）

免疫原

K562 cells expressing the extracellular domain of mouse CD19

应用

in vivo B cell depletion

in vivo CD19 neutralization

in vitro B cell negative selection

Flow cytometry

产品形式

PBS , pH 7.0

Contains no stabilizers or preservatives

内毒素水平

<2eu>

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein G

RRID

AB_10949187

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下，不要冷冻保存。

 

已发表参考文献：

in vivo B cell depletion

Sawen, P., et al. (2016). “Mitotic History Reveals Distinct Stem Cell Populations and Their Contributions to Hematopoiesis.” Cell Rep 14(12): 2809-2818.

Flow cytometry

Becker, A. M., et al. (2015). “ADAM17 limits the expression of CSF1R on murine hematopoietic progenitors.” Exp Hematol 43(1): 44-52 e41-43.

in vitro B cell negative selection

Bouffi, C., et al. (2015). “Transcription Factor Repertoire of Homeostatic Eosinophilopoiesis.” J Immunol 195(6): 2683-2695.

 

详情请咨询 BioXcell 中国代理-上海金畔生物   

Anti-CD261 Reference Antibody(Mapatumumab)

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Anti-CD261 Reference Antibody(Mapatumumab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素：<1 eu>纯度：>95%
别名
TNFRSF10A; TRAILR1
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD261 Reference Antibody(Mapatumumab) is expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

TLR3 激动剂（CD283）及相关产品介绍

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TLR3 激动剂（CD283）及相关产品介绍

TLR3(又名CD283)是一个胞内受体，可识别与病毒感染相关的双链RNA(dsRNA)分子模式。其中一个TLR3的配体，Poly(I:C)，是人工合成的dsRNA类似物。TLR3信号通路不依赖于MyD88，而是通过接头蛋白TRIF(包含TIR结构域的接头蛋白，可诱导IFN-β产生)发挥作用。IRF3(干扰素调节因子3)被磷酸化后导致IFN-β(β干扰素)产生，进一步活化ISRE(干扰素激活反应元件)。另外，TLR3信号通路的激活也可导致AP-1和NF-kB等转录因子入核，促进一些炎症因子的释放。

TLR3激动剂相关产品：

PRODUCT

ORIGIN/DESCRIPTION

GRADE*

WORKING

CONCENTRATION

QTY

CATALOG

CODE

TLR LIGANDS

TLR3 Agonists

Poly(A:U)

Polyadenylic-polyuridylic acid

TLR2 -/ TLR4 –

300 ng- 100 µg/ml

10 mg

tlrl-pau

Poly(I:C) (HMW)

Polyinosine-polycytidylic acid

High molecular weight (1.5-8 kb)

TLR2 -/ TLR4 –

30 ng – 10 µg/ml

10 mg

50 mg

tlrl-pic

tlrl-pic-5

Poly(I:C) (HMW) Biotin

Biotinylated poly(I:C) (HMW)

TLR2 -/ TLR4 –

30 ng – 10 µg/ml

10 µg

tlrl-picb

Poly(I:C) (HMW) Fluorescein

Fluorescein-labeled poly(I:C) (HMW)

TLR2 -/ TLR4 –

10 ng – 10 µg/ml

10 µg

tlrl-picf

Poly(I:C) (HMW) Rhodamine

Rhodamine-labeled poly(I:C) (HMW)

TLR2 -/ TLR4 –

10 ng – 10 µg/ml

10 µg

tlrl-picr

PRR Ligands

PRODUCT

ORIGIN/DESCRIPTION

GRADE*

WORKING

CONCENTRATION

QTY

CATALOG

CODE

TLR3 Agonists

Poly(I:C) (HMW) VacciGrade™

Preclinical grade  poly(I:C) (HMW)

VacciGrade™

10 µg – 100 µg/mouse

10 mg

vac-pic

Poly(I:C) (LMW)

Polyinosine-polycytidylic acid

Low molecular weight (0.2-1 kb)

TLR2 – /TLR4 –

30 ng – 10 µg/ml

25 mg

250 mg

tlrl-picw

tlrl-picw-250

Poly(I:C) (LMW) Rhodamine

Rhodamine-labeled poly(I:C) (LMW)

TLR2 – /TLR4 –

100 ng – 10 µg/ml

10 µg

tlrl-piwr

更多详情请咨询Invivogen代理商-上海金畔生物

InVivoMAb anti-mouse CD40【BE0016-2】

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InVivoMAb anti-mouse CD40【BE0016-2】

克隆号FGK4.5单克隆抗体能与小鼠CD40(也称为Bp50)反应。CD40是一种48 kDa的I型跨膜糖蛋白，属于肿瘤坏死因子受体(TNFR)超家族。CD40广泛表达于抗原呈递细胞(APCs)，如树突状细胞、B细胞、巨噬细胞和单核细胞，以及非免疫内皮细胞、基底上皮细胞和一系列肿瘤。CD40与其配体CD154结合后，作为共刺激分子激活B细胞、树突状细胞、单核细胞和其他APCs。CD40在B细胞活化、分化、增殖和免疫球蛋白同种型转换以及树突状细胞成熟中起作用。激动性CD40单克隆抗体已被证明能激活APCs并促进抗肿瘤T细胞反应。克隆号FGK4.5抗体是一种激动性抗体，已被证明能激活表达CD40的APC。克隆号FGK4.5也可用于体外和体内抑制CD40/CD154相互作用。

产品详情

产品货号

BE0016-2

产品规格

1/5/25/50/100mg

抗体亚型

Rat IgG2a

推荐同型对照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号：BE0089）

推荐抗体稀释液

InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）

免疫原

Recombinant mouse CD40 fusion protein

应用

in vivo CD40 activation

in vitro B cell stimulation/activation

产品形式

PBS, pH 7.0

Contains no stabilizers or preservatives

内毒素水平

<2eu>μ

g)

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein G

RRID

AB_1107647

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下，不要冷冻保存。

 

已发表参考文献：

in vivo CD40 activation

Bauche, D., et al. (2018). “LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis.” Immunity 49(2): 342-352 e345.

in vivo CD40 activation

Bartkowiak, T., et al. (2015). “Unique potential of 4-1BB agonist antibody to promote durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine.” Proc Natl Acad Sci U S A 112(38): E5290-5299.

in vitro B cell stimulation/activation

Xu, H., et al. (2015). “Regulation of bifurcating B cell trajectories by mutual antagonism between transcription factors IRF4 and IRF8.” Nat Immunol.

 

 

详情请咨询 BioXcell 中国代理商-上海金畔生物科技   

Human BTLA/CD272 CHO-S Cell Line

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Human BTLA/CD272 CHO-S Cell Line描述来源应用浓度使用方法储存/保存方法形态外观靶点

产品描述
描述
细胞类型：CHO-S;
载体类型：GSV0
来源
Human
应用
免疫；抗体筛选；体外功能研究
浓度
5E6 cells/vial
使用方法
复苏后培养
储存/保存方法
Liquid nitrogen. 细胞冻存：90% Medium+10% DMSO
形态
液体冻存
基本信息
外观
淡红色
生物活性
靶点
BTLA, CD272

InVivoMAb anti-mouse CD3ε-BioXCell热销产品

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InVivoMAb anti-mouse CD3ε-BioXCell热销产品

克隆号145-2C11单克隆抗体能与小鼠CD3ε反应，CD3ε是一种属于免疫球蛋白超家族的分子量为20 kDa跨膜细胞表面蛋白。CD3ε是结合形成TCR复合物的五条多肽链之一。CD3ε在T淋巴细胞、NK-T细胞和发育中的胸腺细胞上均有不同程度的表达。CD3在TCR信号传导、T淋巴细胞活化和抗原识别中起作用。克隆号145-2C11抗体已被证明通过结合和刺激TCR在体外诱导T淋巴细胞活化、增殖和凋亡。据报道，当在体内使用时，该抗体会产生T细胞活化、无反应或死亡。体内静息T细胞的激活导致细胞因子的释放和随后由T细胞和含Fcγ受体(FcR)的细胞的Ab介导的交联引起的毒性。因此，非FcR结合145-2C 11 f(ab’)2片段更常用于体内应用。据报道，克隆号145-2C11抗体可阻断克隆号17A2抗体与CD3ε+ T淋巴细胞的结合。

 

产品详情

产品货号

BE0001-1

产品规格

1/5/25/50/100mg

抗体亚型

Armenian Hamster IgG1

推荐同型对照

InVivoMAb polyclonal Armenian hamster IgG（货号：BE0091）

推荐抗体稀释液

InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）

免疫原

Mouse BM10-37 cytotoxic T cells

应用

in vitro T cell stimulation/activation

Immunofluorescence

Flow cytometry

Western blot

in vivo T cell depletion

产品形式

PBS, pH 7.0 

Contains no stabilizers or preservatives

内毒素水平

<2eu>

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein A

RRID

AB_1107634

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下，不要冷冻保存。

 

已发表参考文献：

in vivo T cell depletion

Glasner, A., et al. (2018). “NKp46 Receptor-Mediated Interferon-gamma Production by Natural Killer Cells Increases Fibronectin 1 to Alter Tumor Architecture and Control Metastasis.” Immunity 48(1): 107-119 e104.

in vitro T cell stimulation/activation

Lacher, S. M., et al. (2018). “NF-kappaB inducing kinase (NIK) is an essential post-transcriptional regulator of T-cell activation affecting F-actin dynamics and TCR signaling.” J Autoimmun 94: 110-121.

Immunofluorescence

Kim, Y. U., et al. (2015). “Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.” PLoS One 10(3): e0120294.

 

 

详情请咨询 BioXcell 中国代理-上海金畔生物科技   

Rabbit anti-CD19 Monoclonal Antibody(JRMR-266)

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Rabbit anti-CD19 Monoclonal Antibody(JRMR-266)别名宿主反应种属应用分子量纯化方法类型克隆号同种型储存/保存方法背景说明细胞定位UniProt

概述
别名
CD19 抗体;B4; CVID3
宿主
Rabbit
反应种属
Human
应用
WB:1: 2000; IHC-P:1: 200-1: 1000; ICC/IF:1: 50;
分子量
Predicted molecular weight: 61kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
性能
纯化方法
Protein A
类型
Monoclonal Antibody
克隆号
JRMR-266
同种型
IgG
储存/保存方法
储存温度：-20℃，避免反复冻融
靶标
背景说明
作为B淋巴细胞上B细胞抗原受体复合物（BCR）的共同受体发挥作用。降低激活下游信号通路和触发B细胞对抗原反应的阈值
细胞定位
细胞膜
UniProt
P15391